6. Genetic code(遗传密码) Specific nucleotide sequence in the dNa that determining every amino acid The information unit for genetic code is condon(遗传密码的信息单位是密码子) A codon is composed by a triplet(三联体) nucleotides. A codon is described using a three uninterrupted nucleotide sequence non-sense codon qUAAUAGUGA
6. Genetic code(遗传密码) Specific nucleotide sequence in the DNA that determining every amino acid. The information unit for genetic code is condon (遗传密码的信息单位是密码子) A codon is composed by a triplet (三联体)nucleotides. A codon is described using a three uninterrupted nucleotide sequence. non-sense codon (UAA,UAG,UGA)
7. nucleotide Functional unit for genetic--gene Information unit for genetic-codon The minimum unit for mutation and exchanging- nucleotide or base AMP TMP GMP CMP
7. nucleotide Functional unit for genetic——gene Information unit for genetic——codon The minimum unit for mutation and exchanging— — nucleotide or base AMP TMP GMP CMP
2. Plasmid 2.1 definition 明 prokaryote de free from the genome dIehindependent replication (independent replicon独立性的复制子) Jia ccc DNa ( circular covalently closed DNA) dsdNA
2. Plasmid 2.1 definition: prokaryote free from the genome independent replication(independent replicon独立性的复制子) ccc DNA (circular covalently closed DNA) dsDNA
2.2 features: Shape: Linear plasmid later be found in actinomyces such as streptomyces coelicoler(天蓝色链霉菌) and the prokaryote such as Borrelia hermsii(赫氏婢疏螺旋体) Structure: twisted supercoil Size: 1.5-300kb Relative molecular weight: 106-108, =1% genome Funtion: carring non essential genes
2.2 features: Shape:Linear plasmid later be found in actinomyces such as streptomyces coelicoler(天蓝色链霉菌)and the prokaryote such as Borrelia hermsii(赫氏婢疏螺旋体) Structure:twisted supercoil Size:1.5~300kb , Relative molecular weight: 106~108,≈1% genome Funtion:carrring non essential genes
Replicon stringent replication control (1-2 plasmids) relaxed replication control (10-15 plasmids even more) Plasmid curing or elimination 些染料,丝裂霉素C,UV,利福平,重金属离子,Or high tempreture Integration( episome附加体) F facfor
Replicon stringent replication control (1~2 plasmids) relaxed replication control(10~15 plasmids even more) Plasmid curing or elimination: 一些染料, 丝裂霉素C, UV,利福平,重金属离子, or high tempreture Integration (episome附加体) F facfor