Functions of the Major Parts of a Optical Microscope Lamp and Condenser: project a parallel beam of light onto the sample for illumination Sample stage with X-Y movement: sample is placed on the stage and different part of the sample can be viewed due to the X-Y movement capability a Focusing knobs: since the distance between objective and eyepiece is fixed, focusing is achieved by moving the sample relative to the objective lens
Functions of the Major Parts of a Optical Microscope ▪ Lamp and Condenser: project a parallel beam of light onto the sample for illumination ▪ Sample stage with X-Y movement: sample is placed on the stage and different part of the sample can be viewed due to the X-Y movement capability ▪ Focusing knobs: since the distance between objective and eyepiece is fixed, focusing is achieved by moving the sample relative to the objective lens
Light sources Incandescent Light Sources Heat Sink Tungsten Halide La (b) ( Lamp Collector Adjustment Lens Fiber-Optics Lamphouse
Light Sources
Abbe Condenser Optical Pathway Objective Specimen Microscope Abbe Slide Two-Lens Condenser ondenser Aperture Diaphragm Light from the microscope light source Condenser gathers light and concentrates it into a cone of light that illuminates the specimen with uniform intensity over the entire viewfield http://wwyoutube.com/watch?annotationid=annotation100990&feature=iv&srcvid_=l6d3zd2ltsi&v=ntpjuumdxbg-8:08to9:40 http://micro.magnet.fsu.edu/primer/java/kohler/contrast/index.html
Condenser Light from the microscope light source Condenser gathers light and concentrates it into a cone of light that illuminates the specimen with uniform intensity over the entire viewfield http://www.youtube.com/watch?annotation_id=annotation_100990&feature=iv&src_vid=L6d3zD2LtSI&v=ntPjuUMdXbg ~8:08 to 9:40 http://micro.magnet.fsu.edu/primer/java/kohler/contrast/index.html
Specimen Stage X-Y Translation Mechanical Stage Slide Holder Graduated Locator Markings Stage Stage Lock Control Stage Opening X-Y Translation Control Knobs http://micro.magnetfsuedu/primer/flash/stage/index.html
Specimen Stage http://micro.magnet.fsu.edu/primer/flash/stage/index.html
Functions of the Major Parts of a Optical Microscope a Obiective: does the main part of magnification and resolves the fine details on the samples(mo w 10-100) Eyepiece: forms a further magnified virtual image which can be observed directly with eyes(me N 10) a Beam splitter and camera: allow a permanent record of the real image from the objective be made on film (for modern research microscope)
▪ Objective: does the main part of magnification and resolves the fine details on the samples (mo ~ 10 – 100) ▪ Eyepiece: forms a further magnified virtual image which can be observed directly with eyes (me ~ 10) ▪ Beam splitter and camera: allow a permanent record of the real image from the objective be made on film (for modern research microscope) Functions of the Major Parts of a Optical Microscope