Film plane camera black eo Olympus Electrical contacts BX 51 Focusin Transmitted Telescope Automatic Reflected Light Beam Microscope splitter Shutter Light Sensor Reflected light Rubber Extension Projection I Cooling Episcopic ents Lamphouse Eyepiece (Ocular Prisms Shutter Olympus Vertical Illuminator BX51 Dichroic Filters Apertures Collector Research I Breathshield Nose. Microscope Microscope Objective I Mechanical Board Cutaway Condenser Diagram Field Diascopic Diaphragm Transformer Illumination I Intensity Filters Focus Collector Mirror Transmitted, light http://micro.magnetfsu.edu/primer/java/microassembly/index.html
Olympus BX51 Research Microscope Cutaway Diagram Beam splitter camera Reflected light Transmitted light http://micro.magnet.fsu.edu/primer/java/microassembly/index.html
Obiective Lens dmin =0.61/NA Objective specifications Anatomy of an objective Manufacturer. → Screw Thread Lens Aberration Flat-Field Correction Group 3 Correction OLYMPUS Linear Plan Apo Nume rica Aperture Lens Magnification60×/140ol Group 2 DIC H Immersion Specialized Medium Optical ∞/O.17wDo.21 Properties Working Distance Tube Length ens Color Code rou Cover Slip p Thickness Finger Grip Spring-Loaded Front Lens DIC-differential interference contrast Objectives are the most important components of a light microscope: image formation, magnification, the otto. quality of images and the resolution of the microscope ://www.youtubecom/watch?v=p0z4h20stgobjectiveston5:26 ttp//www.youtube.com/watch?v=h8pq9rmuoa8Gradesofobjectiveston2:30&3:25-4:50 ttps://www.youtube.com/watch?v=fwBjpi8cklyAlignmentofOm
Objective specifications Objectives are the most important components of a light microscope: image formation, magnification, the quality of images and the resolution of the microscope Objective Lens Anatomy of an objective rical ture dmin = 0.61l/NA http://www.youtube.com/watch?v=P0Z4H2O_Stg Objectives to~5:26 http://www.youtube.com/watch?v=H8PQ9RMUoA8 Grades of objectives to~2:30 & 3:25-4:50 https://www.youtube.com/watch?v=FwBjpi8ck1Y Alignment of OM DIC-differential interference contrast
Defects in lens Curvature of field When visible light Focal Point 11 12 is focused through a Curvature curved lens the mage plane produced by the lens will be curved a the image appears Focal sharp and crisp Plane either in the center or on the edges of the viewfield but not both http://micro.magnetfsu.edu/primer/java/aberrations/curvatureoffield/index.html
▪ Curvature of Field - When visible light is focused through a curved lens, the image plane produced by the lens will be curved ▪ The image appears sharp and crisp either in the center or on the edges of the viewfield but not both Defects in Lens http://micro.magnet.fsu.edu/primer/java/aberrations/curvatureoffield/index.html
Defects in lens Chromatic Aberration Chromatic Aberration Axial - blue light is refracted to the greatest extent followed byWhite BI ue Light\ Red green and red light a phenomenon commonly referred to as dispersion reen Lateral-chromatic difference of magnification: the blue image of a detail was slightly larger than the Achromat Doublet green image or the red image in white light thus causing color Focal Points ringing of specimen details at the outer regions of the field of view A converging lens can be combined Red with a weaker diverging lens, so that Green and the chromatic aberrations cancel for Blue certain wavelengths: weaker diverging lens The combination- achromatic doublet http://www.youtube.com/watch?v=yh7rbru7av8&list=pl02d1d436a44b521achromaticaberration
▪ Chromatic Aberration ▪ Axial - Blue light is refracted to the greatest extent followed by green and red light, a phenomenon commonly referred to as dispersion ▪ Lateral - chromatic difference of magnification: the blue image of a detail was slightly larger than the green image or the red image in white light, thus causing color ringing of specimen details at the outer regions of the field of view Defects in Lens A converging lens can be combined with a weaker diverging lens, so that the chromatic aberrations cancel for certain wavelengths: The combination – achromatic doublet weaker diverging lens http://www.youtube.com/watch?v=yH7rbRu7Av8&list=PL02D1D436A44B521A chromatic aberration http://www.youtube.com/watch?v=H8PQ9RMUoA8 at~3:30-4:30
Eyepiece Lens Aberration-Free 10X Eyepiece With Diopter Adjustment Rubber Eyecup Evelens Single Lens Diopter Adjustment : Lens ole Apertur Eyetube (Diaphragm) Fastening UW10xA/24 Field Lens Screw Doublet Eyetube Mounting Eyetube Flange Insert Housing M=(L/fo)(25/fe) Eyepieces(Oculars) work in combination with microscope objectives to further magnify the intermediate image http://micro.magnetfsuedu/primer/anatomy/oculars.html http://www.birdwatchingcom/optics/diopter_set.html
(Diaphragm) Eyepieces (Oculars) work in combination with microscope objectives to further magnify the intermediate image Eyepiece Lens M=(L/fo)(25/fe) http://micro.magnet.fsu.edu/primer/anatomy/oculars.html http://www.birdwatching.com/optics/diopter_set.html