Axial resolution- Depth of Field Depth of focus (f mm) Depth of Field ranges(F um) Low Depth of focus Image plane High object specimen Plane NA fF 0.10.1315.5Deph 04385.8 of Field 9580.00.19 Object plane The distance above and below The axial range through which geometric image plane within an object can be focused without which the image is in focus any appreciable change in image MI NAIf F sharpness F is determined by Na http://www.matter.org.uk/tem/depth_of_field.htm http://www.youtube.com/watch?v=fvc2wluqeugatw3:40
Depth of focus (f mm) The distance above and below geometric image plane within which the image is in focus The axial range through which an object can be focused without any appreciable change in image sharpness (F m) M NA f F M NA f F Axial resolution – Depth of Field Depth of Field Ranges (F m) F is determined by NA. NA f F 0.1 0.13 15.5 0.4 3.8 5.8 .95 80.0 0.19 http://www.matter.org.uk/tem/depth_of_field.htm http://www.youtube.com/watch?v=FvC2WLUqEug at~3:40
Depth of Focus The distance above and below geometric image plane within which the image is in focus CCD camera Camera Tube Eye Observation Light Source Tube Lens Microscope Optical Objective Train Components Condenser Aperture aphragm Condenser Field Diaphragm Figure 2
Depth of focus (f mm) Depth of Focus CCD camera The distance above and below geometric image plane within which the image is in focus
Axial resolution Depth of Field The axial range through which an object can be focused without any appreciable change in image sharpness Depth of Field Ranges 寄 ject lane 25um Small Large F
Depth of focus (f mm) Axial resolution – Depth of Field NA f F 0.1 0.13 15.5 0.4 3.8 5.8 .95 80.0 0.19 Small F Large F The axial range through which an object can be focused without any appreciable change in image sharpness. 25m
Basic components and their functions http://www.youtube.com/watch?v=rka8_mif6-e Microscope Review(simple, clear) http://www.youtube.com/watch?v=b2pc]5s-iyk Microscope working in animation(How to use a microscope) http://www.youtube.com/watch?annotation_id=annotation_100990&featur e=iv&src_vid=L6d3zD2LtsI&v=ntPjuUMdXbg(I http://www.youtube.com/watch?v=vqtmhj3valg(ii) Parts and Function of a Microscope(details) http://www.youtube.com/watch?v=x-w98ka8uqu&feature=related How to use a microscope(specimen preparation at 1: 55-2: 30) http://www.youtube.com/watch?v=bgbgablev4g How to care for and operate a microscope
Basic components and their functions http://www.youtube.com/watch?v=RKA8_mif6-E Microscope Review (simple, clear) http://www.youtube.com/watch?v=b2PCJ5s-iyk Microscope working in animation (How to use a microscope) http://www.youtube.com/watch?annotation_id=annotation_100990&featur e=iv&src_vid=L6d3zD2LtSI&v=ntPjuUMdXbg (I) http://www.youtube.com/watch?v=VQtMHj3vaLg (II) Parts and Function of a Microscope (details) http://www.youtube.com/watch?v=X-w98KA8UqU&feature=related How to use a microscope (specimen preparation at~1:55-2:30) http://www.youtube.com/watch?v=bGBgABLEV4g How to care for and operate a microscope
Eyepiece Hooke Microscope circa 1670 sk Bearer Basic components and their functions Focusing Screw ctive Figure 1 Specime 1) Eyepiece(ocular lens) 2)Revolving nose piece(to hold multiple objective lenses) (3 Objective lenses (4)And(5)Focus knobs (4)Coarse adjustment Fine adjustment (6 Stage(to hold the specimen) 7) Light source (lamp 8) Condenser lens and diaphragm (9) Mechanical stage(move the specimen on two horizontal axes for positioning the specimen
Basic components and their functions (1) Eyepiece (ocular lens) (2) Revolving nose piece (to hold multiple objective lenses) (3) Objective lenses (4) And (5) Focus knobs (4) Coarse adjustment (5) Fine adjustment (6) Stage (to hold the specimen) (7) Light source (lamp) (8) Condenser lens and diaphragm (9) Mechanical stage (move the specimen on two horizontal axes for positioning the specimen)