WhyisiPSCellResearchsoInteresting?Mousewith8sickle cell anemiaTransplantCollectskincells2DifferentiateintobloodstemcellsRedBloodCells6GeneticallyRecoveredmousecorrectediPScellsOct4,Sox2KIf4,c-Myc3ReprogramVirusesintoES like-iPScellsCorrectmutation5GeneticallyidenticaliPScellsMlustrationbyTomDiCesare4
Why is iPS Cell Research so Interesting? 1 2 3 4 5 6 7 8
ThePotential ofiPSResearchNormalembryodevelopment·SkinfibroblastsDiseasemodels·BloodcellsDrug screening·Hairkeratinocytes??cell therapyALSAATAizheimer's00Cystic FibrosisDiabetes3IBDParkinson'sCancerOct4cMyoK1f4Sox2Transduction/TransfectioniPScellsALS:Amyotrophiclateralsclerosis;IBD:Inflammatoryboweldisease
ALS: Amyotrophic lateral sclerosis; IBD: Inflammatory bowel disease
Thecell cycle,orcell-divisioncycle,istheseries of events that take place in a cell leading toitsdivisionandduplication(replication)
MitoticDivisionofCellThochromatidsare+cytoplasmheldtogetheratthecentromere+nucleuschromosamereplicationtchromosomosalignmentandseparationofchromatids;thenuclearcentromoremombranedisappearschromatidchromatid川spindles Irom centriolespull the chromatidsapar,Centriolesarepartofcelfscentrosomos.centriolecytokinesisMITOTICDIVISION OF CELL
Mitotic Division of Cell
ChromosomeDAdoubehetsmest,mais a double-stranded helical业业业structureofDNA2DNAis complexedTelomeres3Eachnucleosomeconsistsof4Achromatosome consistswithhistonestoeighthistoneproteinsaroundofanudeosome plusthewhichtheDNAwraps1.65times.H1histone.form nudeosomesHistoneHIOrange-coloredNucleosomecore ofregions indicateeighthistonemoleculesCentromer11nmheterochromatin,G..thatformsloopsaveragingChromatosome5Thenuclecsomesfoldup1o300nminlengthproducea30-nmfiber..Euchromatin30nm7The300-nm fibersarecompressedandfoldedto8Tightcollngofthe250-nm250-nm-widefberproducea 250-nm-widefiberfiberproducesthechromatidofachromosome71400nm700nmAchromosome is anorganized structureof DNA andproteinfoundin cells.It is asinglepiece of coiledDNA containingmanygenes,regulatoryelementsandothernucleotidesequences.ChromosomesalsocontainDNA-boundproteins,whichservetopackagetheDNAandcontrolitsfunctions
Chromosome