22.3 Nuclear splicing proceeds through a lariat Lariat is an intermediate in RNA Splicing in which a circular structure with a tail is created by a 5 -2' bond 消当
Lariat is an intermediate in RNA splicing in which a circular structure with a tail is created by a 5′-2′ bond. 22.3 Nuclear splicing proceeds through a lariat
5 site 3 site 22.3 Nuclear splicing GU UACUAAC AG proceeds through a lariat y8ON PY80 PY87 Pu75 A Ry95 Animal consensus Cut at 5 site &form lariat by 5-2 bond conne cting the intron 5 -G to the 2 'of a at the branch site Figure 22.6 Splicing occurs in UACUAACAG two stages. in Cut at 3 site join exons: intron re le ased as lariat which the5￠ VACUA exon is separated an en IS joined to the3￠exon Debranch introl 消当 UACUAAC A
Figure 22.6 Splicing occurs in two stages, in which the 5￠ exon is separated and then is joined to the 3￠ exon. 22.3 Nuclear splicing proceeds through a lariat
22.3 Nuclear splicing proceeds through a OH lariat EX-P最AOP正XCn2 Figure 22.7 Nuclear splicing occurs by two Exon- OH transesterification SUC reactions in which 知 Aus. OP-Ekon a free OH end attacks a phosphodiester pl Exn①Pxn2 bond 消当 CIREILAIIEEr-OH
Figure 22.7 Nuclear splicing occurs by two transesterification reactions in which a free OH end attacks a phosphodiester bond. 22.3 Nuclear splicing proceeds through a lariat
22.4 The spliceosome contains SnRNAS SCRNA is any one of several small cytoplasmic RNA molecules present in the cytoplasm and (sometimes) nucleus SnRNA(small nuclear RNa) is any one of many small RNA species confined to the nucleus several of the snrnas are involved in splicing or other rna processing reactions 消当
scRNA is any one of several small cytoplasmic RNA molecules present in the cytoplasm and (sometimes) nucleus. snRNA (small nuclear RNA) is any one of many small RNA species confined to the nucleus; several of the snRNAs are involved in splicing or other RNA processing reactions. 22.4 The spliceosome contains snRNAs
22.4 The spliceosome Domain D UC contains SnRNas sm binding Domain C Figure 22. 8UI A snrna has a base CG paired structure Domain B that creates several ACcU domains.The5￠ GGAUG GCI CCUAU CGG GGGACOUGC UU GACGGUCCAU UCA UApppGme evon GUAA GUA itron end remains single stranded and can Intron pairing base pair with the 5￠ splicing site Gc&c 消当 Domain A
Figure 22.8 U1 snRNA has a base paired structure that creates several domains. The 5￠ end remains single stranded and can base pair with the 5￠ splicing site. 22.4 The spliceosome contains snRNAs