SCC of the Anal CanalHistology: keratinizing, nonkeratinizing(transitional) and basaloidAnal canal is 5 times more common than analmarginIncidenceis 1/10thatof rectalcancerTransformationZone:dentate line(Transitionalurothelium-like)The most common presenting symptom isbleeding>50% of patients withanal painA small number of patients will be asymptomatic Most patients are diagnosed late stage
SCC of the Anal Canal • Histology: keratinizing, nonkeratinizing (transitional) and basaloid • Anal canal is 5 times more common than anal margin • Incidence is 1/10 that of rectal cancer • Transformation Zone: dentate line (Transitional urothelium-like) • The most common presenting symptom is bleeding • >50% of patients with anal pain • A small number of patients will be asymptomatic • Most patients are diagnosed late stage
Molecular diagnosis of HPV Hybrid Capture Il-Qiagen (Digene)-HR and LR CervistaHologic (Thirdwave Technology)- HR and reflex to 16/18RocheAmplicorPCRRoche-HR and 16/18·PCR/linearprobearray-Roche- 37 HPV typesIn situ hybridization
Molecular diagnosis of HPV • Hybrid Capture II—Qiagen (Digene) – HR and LR • Cervista—Hologic (Thirdwave Technology) – HR and reflex to 16/18 • Roche Amplicor PCR—Roche – HR and 16/18 • PCR/linear probe array—Roche – 37 HPV types • In situ hybridization