"N"for normal soybeanthin-layer chromatography"s" for sugar standards(TLC)"H" for high sucrose soybean5cmx10cmplate1.5cmcmcmNSH15mm11
"N""for"normal"soybean" "S""for"sugar"standards" "H""for"high"sucrose"soybean" 11" thin<layer&chromatography& (TLC)&
SucroseRaffinoseStachyose70%isopropylalcohol91%isopropylalcohol12http://www.biotech.iastate.edu/lab_protocols/HSSB-TLCStudent.html
12" hIp://www.biotech.iastate.edu/lab_protocols/HSSBPTLC_Student.html
OHOHMeOHHOHOOHHCZnCl2HCOHOHOMeFischerglycosidation/glycosylationEquilibriumreactionTreated with aqueous acid can reverse the reactionSource of anhydrous acid: Bronsted acid/Lewis acidUnderthermodynamiccontrolStable under almost all other conditions except aqueous acidPROTECTIONOFANOMERICCENTER13
Fischer"glycosida:on/glycosyla:on" Source"of"anhydrous"acid:"Bronsted"acid/Lewis"acid" Equilibrium"reac:on"" Treated"with"aqueous"acid"can"reverse"the"reac:on" Under"thermodynamic"control" Stable"under"almost"all"other"condi:ons"except"aqueous"acid" 13