Lecture 13 Effects of glycosylationon protein structure and function
Lecture'13'Effects'of'glycosyla3on' on'protein'structure'and'func3on
Learning objects· Experimental approaches to studying theeffects of glycans on protein structure andfunction: How glycans affect protein folding andstability: The effects of glycans on interactions ofproteins
Learning'objects' • Experimental'approaches'to'studying'the' effects'of'glycans'on'protein'structure'and' func3on' • How'glycans'affect'protein'folding'and' stability' • The'effects'of'glycans'on'interac3ons'of' proteins
. In manyinstances,glycosylation directlyaffects the properties of proteins andmembranes, sometimes with importantbioogical consequences: The examples illustrate some of the ways inwhich glycoprotein functions are modulatedby glycosylation, usually by affecting theirstability or surfaceproperties
• In'many'instances,'glycosyla3on'directly' affects'the'proper3es'of'proteins'and' membranes,'some3mes'with'important' bioogical'consequences' • The'examples'illustrate'some'of'the'ways'in' which'glycoprotein'func3ons'are'modulated' by'glycosyla3on,'usually'by'affec3ng'their' stability'or'surface'proper3es
1 Various approaches can be used tostudy the effects of glycosylation
1 Various approaches can be used to study the effects of glycosylation
Altering glycosylation to determinewhat effect the alternation haseliminationof all sugarsfromall theattachmentsites on the protein. Elimination of sugars at specific attachment sites.Removalof portions of the sugar structures· N-linked sugars can be removed completely fromglycoproteins by TfOH or anhydrous HF
Altering'glycosyla3on'to'determine' what'effect'the'alterna3on'has • elimina3on'of'all'sugars'from'all'the'aEachment' sites'on'the'protein' • Elimina3on'of'sugars'at'specific'aEachment'sites' • Removal'of'por3ons'of'the'sugar'structures' • NJlinked'sugars'can'be'removed'completely'from' glycoproteins'by'TfOH'or'anhydrous'HF