1. N-linked glycosylation
1.#N%linked#glycosyla2on#
Learning objectives. The structures of different classes of N-linkedglycans. Chemical steps in the biosynthesis of N-linkedglycans. the cellular compartmentalization biosyntheticsteps: The nature and causes of heterogeneity of N.linked glycans
Learning#objec2ves# • The#structures#of#different#classes#of#N%linked# glycans • Chemical#steps#in#the#biosynthesis#of#N%linked# glycans • the#cellular#compartmentaliza2on#biosynthe2c# steps# • The#nature#and#causes#of#heterogeneity#of#N% linked#glycans
: The N-linked glycosylation pathway is the bestunderstood route to protein glycosylation.. This pathway can be used to illustrate generalprinciples of glycoconjugate structure andbiosynthesis.: The nature of the N-linked glycosylationpathway also provides some insight into thefunctions and evolutionary history
• The#N%linked#glycosyla2on#pathway#is#the#best# understood#route#to#protein#glycosyla2on.# • This#pathway#can#be#used#to#illustrate#general# principles#of#glycoconjugate#structure#and# biosynthesis.# • The#nature#of#the#N%linked#glycosyla2on# pathway#also#provides#some#insight#into#the# func2ons#and#evolu2onary#history#
1.1 Diverse N-linked glycans have acommoncorestructure
1.1 Diverse N-linked glycans have a common core structure
Asparagine(Asn)GlcNAcOHresidueinpolypeptideHCH2H.ONH0IHOGlcNAcβ1-AsnNH-C-CH2-CHHOBHNHH1C=OHC=OHighmannoseManaα1-2Manα16Mana1ManB1-4GlcNAcβ1-4GlcNAcβ1-AsnMang1-2Mang12Mana1Manα1-2Manα1Complex:bi-antennaryNeuAcα2-6Galβ1-4GlcNAcβ1-2Manα16Manβ1-4GlcNAcβ1-4GlcNAcβ1-Asn3NeuAcα2-6Galβ1-4GlcNAcβ1-2Manα1
GlcNAcβ1%Asn#