上泽充豆大学 Shanghai Jiao Tong University Staining:Increasing Contrast for Bright-Field Microscopy SHA 8 染色：增加明视野显微镜的对比度 AUIAO TONG UNA
Staining: Increasing Contrast for Bright-Field Microscopy 染色: 增加明视野显微镜的对比度
上泽充豆大学 Shanghai Jiao Tong University Staining:Increasing Contrast for Bright-Field Microscopy Staining:Increasing Contrast for Bright-Field Microscopy Staining:Increasing Contrast for Bright-Field Microscopy Shanghai Jiao Tong University
Shanghai Jiao Tong University Staining: Increasing Contrast for Bright-Field Microscopy Staining: Increasing Contrast for Bright-Field Microscopy Staining: Increasing Contrast for Bright-Field Microscopy
Figure 2.3 I.Preparing a smear Steps of Spread culture in thin Dry in air film over slide staining Il.Heat fixing and staining cells for microscopic observation Pass slide through Flood slide with stain; flame to heat fix rinse and dry Ill.Microscopy 100× Slide Oil Place drop of oil on slide; examine with 100x objective lens
Figure 2.3 I. Preparing a smear II. Heat fixing and staining III. Microscopy Spread culture in thin film over slide Pass slide through flame to heat fix Dry in air Flood slide with stain; rinse and dry Place drop of oil on slide; examine with 100 objective lens Slide Oil Steps of staining cells for microscopic observation
上商充通大学 Shanghai Jiao Tong University T Differential Stains:The Gram Stain SHANG 鉴定染色：革兰氏染色法 AIJIAO TONG UNI
Differential Stains: The Gram Stain 鉴定染色: 革兰氏染色法
Figure 2.4 Step 1 Flood the heat-fixed smear with crystal Result: violet for 1 min All cells purple Step 2 Add iodine solution for 1 min Result: All cells remain purple Step 3 Decolorize with alcohol briefly Result: 一about20sec Gram-positive cells are purple; gram-negative cells are colorless Step4 Counterstain with Result: safranin for 1-2 min Gram-positive (G)cells are purple; gram-negative(G-)cells are pink to red (a) FLASH (c)
Figure 2.4 Step 1 Step 2 Step 3 Step 4 Result: All cells purple Result: All cells remain purple Result: Gram -positive cells are purple; gram -negative cells are colorless Result: Gram -positive (G + ) cells are purple; gram -negative (G - ) cells are pink to red Flood the heat -fixed smear with crystal violet for 1 min Add iodine solution for 1 min Decolorize with alcohol briefly — about 20 sec Counterstain with safranin for 1 –2 min G + G - Leon J. Lebeau Molecular Porobes, Inc., Eugene, Oregon FLASH