Restriction enzyme digestion of DNA dsDNA 5-NNNNNNNNGAAT TCNNNNNNNNNNN -3 A sequence 3-NNNNNNNNCT TAAGNNNNNNNNNNN -5 recognized by EcoRI 5'-NNNNNNNNG AAT TCNNNNNNNNNNN -3 Cut 3-NNNNNNNNCT TAA GNNNNNNNNNNN -5
Restriction enzyme digestion of DNA 5’-NNNNNNNNGAAT TCNNNNNNNNNNN -3’ 3’-NNNNNNNNCT TAAGNNNNNNNNNNN -5’ dsDNA A sequence recognized by EcoRI Cut 5’-NNNNNNNNG 3’-NNNNNNNNCT TAA AAT TCNNNNNNNNNNN -3’ GNNNNNNNNNNN -5’
Type II restriction enzymes Palindrome sequences: 5-CTCGAG-3' 3'-GAGCTC-5 Cut to produce 5-CTA6-3→ 5-C O p TCGAG-3 Sticky ends: 3"-GACCTC-5'Xhol 3'-GAGCT Po C-5' or Blunt ends: 5'-CTCGAG-35-CTCGAG-3 3-GACCTC-5 ScI3'-GAGCTC-5°
Type II restriction enzymes Palindrome sequences: Cut to produce Sticky ends: or Blunt ends: XhoI SciI