多尺度相关显微分析的必要性 O TONG 以电沉积金属镍中的非正常晶粒长大为例： 1.晶粒尺寸(OM) {100} 2.非正常晶粒长大特征(SEM) 晶面 3.晶界特征(TEM) EBSD 光学显微镜 SEM/EBSD TEM/STEM/EDS √晶粒尺寸分布 √晶粒取向分布 √晶界/晶粒内成分分布 50μm :10μm 1μm Ni K Ni K Ni KI S Ko 特征定位 200 Sulfur rich phase School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering 以电沉积金属镍中的非正常晶粒长大为例： 1. 晶粒尺寸（OM） 2. 非正常晶粒长大特征（SEM） 3. 晶界特征（TEM） 1 μm 光学显微镜 晶粒尺寸分布 50 μm 10 μm TEM/STEM/EDS 晶界/晶粒内成分分布 特征定位 SEM/EBSD 晶粒取向分布 Ni_50_500°C_1 h, matrix grain size 1.24µm large grain size 14.96 µm Ni_50_500°C_10 h, matrix grain size 2.38µm large grain size 181.88µm EBSD {100} 晶面 Sulfur-rich phase 多尺度相关显微分析的必要性
Correlative Light and Electron Microscopy (CLEM) In an integrated CLEM system,the sample is imaged using an electron beam and an optical light path simultaneously. Fluorescence microscope- highlight the regions of interest Electron microscope-obtain structural information at the nano- scale Carolien van Rijnsoever,Viola Oorschot Judith Klumperman,Nature Methods 5,973-980(2008) Pascal de Boer,Jacob P Hoogenboom Ben N G Giepmans,Nature Methsz,5(2015) School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering Correlative Light and Electron Microscopy (CLEM) • In an integrated CLEM system, the sample is imaged using an electron beam and an optical light path simultaneously. Carolien van Rijnsoever, Viola Oorschot & Judith Klumperman, Nature Methods 5, 973 - 980 (2008) Pascal de Boer, Jacob P Hoogenboom & Ben N G Giepmans, Nature Methods 12, 503–513 (2015) Electron microscope- obtain structural information at the nanoscale Fluorescence microscopehighlight the regions of interest
GFP-标记的NS5A(一 501 10m b 种亚基因) NS5A-GFP信号主要对 应于LDs和聚集在其附近 的DMV LD DMVs http://www.imaging-git.com/science/electron- and-ion-microscopy/correlative-light-and- 200nm electron-micro$ce砂学与工程学院 School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering http://www.imaging-git.com/science/electronand-ion-microscopy/correlative-light-andelectron-microscopy GFP-标记的NS5A（一 种亚基因） NS5A-GFP信号主要对 应于LDs和聚集在其附近 的DMV
lo TON Part 2 CORRELATIVE MULTI-SCALE CHARACTERIZATION 材料科学与工程学院 School of Materials Science and Engineering
材料科学与工程学院 School of Materials Science and Engineering CORRELATIVE MULTI-SCALE CHARACTERIZATION Part 2
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材料科学与工程学院 School of Materials Science and Engineering 12 225 kW 60 kW 动能回收电机：120 kW 热能回收电机：90 kW