Background( ncRNA分类) 依据 ncrNA表达特点及功能: 1.组成型 ncRNA( constitutive ncRNA 2调节型 MerNA( regulatory ncRNA) ☆依据 ncRNA分子大小(含碱基数量的多少): 1.小分子 ncrNA( small non- coding rna) 18~200nt 2长链 ncrNA( long non-coding rna, Incana >200nt~100kb
Background（ncRNA分类） ❖依据ncRNA表达特点及功能： 1. 组成型ncRNA(constitutive ncRNA) 2. 调节型ncRNA(regulatory ncRNA) ❖依据ncRNA分子大小（含碱基数量的多少） : 1. 小分子ncRNA（small non-coding RNA): 18~200nt 2.长链ncRNA（long non-coding RNA, lncRNA): ≥200nt~100kb
Background(小分子nRNA种类) Subclasses Symbol Transfer RNAs tRNAs MicroRNAs miRNAs Ribosomal 5s and 5.85 RNAs TRNAs Pwi interacting RNAs piRNAS Tiny transcription initiation RNAS trNAs Small interfering RNAs Promoter-associated short RNAs PASRs Termini-associated short rNAs TASR Antisense termini assocated short rNas aTAS Small ncRNA (18 to 200 nt in size) mall nucleolar RNAs snoRNAs Transcription start site antisense rNas [SSa-RNAs mall nuclear RNAs snRNA Retrotransposon derived RNAs RE RNAS 3 UTR derived刚NAs paRNAs X ncRNA x-ncRNA Human Y RNA hY RNA Unusually small RNAS sRNAS Small NP9O-assodiated RNAs sarS Vault rNAs vtRNAS
Background（小分子ncRNA种类）
Background( IncaNA种类) Type Subclasses Symbol Ribosomal 18S and 28S RNAS TRNAS Long or large intergenic ncRNAS lincRNAS Transcribed ultraconserved UCRS pseudogenes none GAA-repeat containing RNAS GRC-RNAS Long ncRNA (nCRNAS, 200 nt to >100 kb in size) Long intronic ncRNAS Antisense RNAs Promoter-associated long RNAs PALRS Promoter upstream transcripts PROMPTS Stable excised intron rnas none Long stress-induced non-coding transcripts LSINCTS
Background（lncRNA种类）
Background Non coding RNA (ncRNA) Protein 15% of genome,18258(假基因12134) Small ncrNa:0.01% of genome,≈1000(≤200nt) Long ncrna 91 of genome , >100,000
◆Non coding RNA (ncRNA) Protein： 1.5% of genome，18258(假基因12134） Small ncRNA ：0.01% of genome ，≈1000 (<200nt) Long ncRNA ： 91% of genome ，>100,000 Background
Background (SiRNA) SiRNA:小干扰RNA( small interference rna) 令生成过程及作用:长双链RNA被细胞内 Dicer( RNasel 家族中的特异性双链RNA内切酶切割为21~25bp的短 双链RNA,称为 SirNA;随后 siRNA与细胞内的某些 酶和蛋白质形成复合体,称为RNA诱导的沉默复合体 (RNA- induced silencing complex,RISC)。RISC可识 别与 sirNA有同源序列的mRNA,进而在特异位点将 mRNA切断或抑制蛋白质翻译
❖ siRNA：小干扰RNA（small interference RNA） ❖ 生成过程及作用：长双链RNA被细胞内Dicer(RNaseⅢ 家族中的特异性双链RNA内切酶)切割为21～25bp的短 双链RNA，称为siRNA；随后siRNA与细胞内的某些 酶和蛋白质形成复合体，称为RNA诱导的沉默复合体 （RNA-induced silencing complex,RISC）。RISC可识 别与siRNA有同源序列的mRNA，进而在特异位点将 mRNA切断或抑制蛋白质翻译。 Background（siRNA）