动物转基因技术 CONSTRUCTION OF A TRANSGENIC MOUSE Transgene Fertile ATG TAA ATT Collection of Injection of Transgene Fertilized Eggs into male Pronucleus Sterile Implant Injected Embryos into Pseudopregnant females Founder Animal Live Birth Test for Transgene ARH Lab
动物转基因技术
吸引眼球的“人兽杂交”新闻：转入人类基因的动物 HUMAN GROWTH HORMONE GENE MT PROMOTER PBR 1983年《科学》杂志刊登首例人鼠转基因，人生长素转入小鼠。 正常小鼠（左）和转人生长素小鼠（右）。圆环是转入的DNA结构
吸引眼球的“人兽杂交” 新闻：转入人类基因的动物 1983年《科学》杂志刊登首例人鼠转基因，人生长素转入小鼠。 正常小鼠（左）和转人生长素小鼠（右）。圆环是转入的DNA结构
基因重组技术：打破种属壁垒的“挖墙角”和“拉郎配” 天然重组：跳跃的基因 人工重组：任何基因 Small spots: frecuent excision Large spot: late in kemel excision early in developrent kernal development Restriction Foreign DNA Vector Enzyme Fragments Restriction Enzyme Revertant: element No excision Cut excised autonomous element not Joining of Cohesive Ends Plasmid L其511 restcred in genome No product Activator TE Pigment gene Excision of TE in somatic cells spot Recombinant n germ cells revertant Plasmid 转药物基因的山羊 Atryn-抗凝血 2022-3-11 18
2022-3-11 1 18 基因重组技术：打破种属壁垒的“挖墙角”和“拉郎配” 天然重组：跳跃的基因 人工重组： 任何基因 转药物基因的山羊 Atryn-抗凝血
>植物转基因技术与高效农业育种： 目的基因选择与重组 愈伤组织培养 白aoNW3 抗除草剂基因 Plant Breeding DNA分离基因扩增 (PCR技术) 基因克隆与表达载体 引入细胞 0000000000c
Ø植物转基因技术与高效农业育种： 目的基因选择与重组 愈伤组织培养 抗除草剂基因 DNA分离基因扩增 （PCR 技术） 基因克隆与表达载体 引入细胞
>人与转基因：植物转基因技术 GENE identified and isolated B Agrobacterium Gene Gun gene gene inserted replication bacterium mixed into ti plasmid with plant cells coated with DNA gold particles ti plasmid moves cells shot with gene into plant cell and gun and DNA inserts DNA into incorporated into plant chromosome plant cell chromosome Screening for cells with transgene 厦液风 cells screened transformed cells transgenic plant for transgene selected with regenerated from single selectable marker transformed cell
Ø人与转基因：植物转基因技术