The Measurement of MicrobialConcentrations Cell ConcentrationTurbidity of a culture, measured by photoelectricmeansThe viable cell count is usually considered themeasure of cell concentrationBiomass Density+ determining the dry weight of a microbial cultureafter it has been washed with distilled waterIn practice, the investigator customarily preparesastandard curve that correlates dry weight withturbiditySHIHEZI UNIVERSITY
The Measurement of Microbial Concentrations Cell Concentration Turbidity of a culture, measured by photoelectric means The viable cell count is usually considered the measure of cell concentration Biomass Density determining the dry weight of a microbial culture after it has been washed with distilled water In practice, the investigator customarily prepares a standard curve that correlates dry weight with turbidity SHIHEZI UNIVERSITY
Measuring bacterial masss (live +dead) in ligquid cultureTurbidity(Cloudiness)SHIHEZI UNIVERSITY
Measuring bacterial mass (live + dead) in liquid culture Turbidity Turbidity (Cloudiness) (Cloudiness) SHIHEZI UNIVERSITY
Measuring viable bacteriaColony forming unitscolonySHIHEZI UNIVERSITY
Measuring viable bacteria colony colony Colony forming units Colony forming units SHIHEZI UNIVERSITY
Bacteria MultiplicationCell all-Plasma membraneChromosomeBinary fission2insocia苗刻SHIHEZI UNIVERSITY
Bacteria Multiplication Binary fission G- 菌分 分 裂 SHIHEZI UNIVERSITY
The Growth CurveTable4-2. Phasesof microbial growth curveoieSectionofPhaseCurveGrowthRateZeroALagBBAccelerationIncreasingCExponentialConstantDRetardationDecreasingLLMaximum stationaryZeroTimeLLDeclineNegative death)Figure 4-2. Cell oncentration curve.A: The Lag PhaseE: The Maximum Stationary PhaseC: The Exponential PhaseF: The Death Phase (Decline PhaseSHIHEZI UNIVERSITY
The Growth Curve A: The Lag Phase E: The Maximum Stationary Phase C: The Exponential Phase F: The Death Phase (Decline Phase) SHIHEZI UNIVERSITY