12.4 Each eukaryotic UNIDIRECTIONAL REPLICATION chromosome contains many replicons BIDIRECTIONAL REPLICATION Figure 12.5 Different densities of radioactive labeling can be used to distinguish Heavy density label unidirectional and Light density label bidirectional replication Not labeled (invisible on autoradiograph) 消当
Figure 12.5 Different densities of radioactive labeling can be used to distinguish unidirectional and bidirectional replication. 12.4 Each eukaryotic chromosome contains many replicons
12.4 Each eukaryotic chromosome contains Simple Y Double Y Bubble Asymmetr ic bubble many replicons Figure 12.6 The position of dougles during the origin and the number replication of replicating forks << determine the shape of a replicating restriction fragment, which can be Second dimension exaggerates followed by its contribution of 3D shap 2 kh electrophoretic path(solid 1 kb 1 kb 1 kb line ). The dashed line shows the path for a linear First dimension separates byy DNA 消当
Figure 12.6 The position of the origin and the number of replicating forks determine the shape of a replicating restriction fragment, which can be followed by its electrophoretic path (solid line). The dashed line shows the path for a linear DNA. 12.4 Each eukaryotic chromosome contains many replicons
12.4 Each Origin 1 Origin 2 eukaryotic MMMMNA chromosome contains many NN MMM replicons Figure 12.8 NV Measuring the size wv 八AA of the replicon requires a stretch Measure average replicon length of dna in which adjacent replicons AMMM Fused replicons are active 消当
Figure 12.8 Measuring the size of the replicon requires a stretch of DNA in which adjacent replicons are active. 12.4 Each eukaryotic chromosome contains many replicons
12.4 Each eukaryotic chromosome contains many replicons Figure 12. 9 Replication forks are organized into foci in the nucleus. Cells were labeled with BrdU The leftmost panel was stained with propidium iodide to identify bulk DNA. The right panel was stained using an antibody to brdu to identify replicating DNA 消当
Figure 12.9 Replication forks are organized into foci in the nucleus. Cells were labeled with BrdU. The leftmost panel was stained with propidium iodide to identify bulk DNA. The right panel was stained using an antibody to BrdU to identify replicating DNA. 12.4 Each eukaryotic chromosome contains many replicons
12. 4 Each eukaryotic chromosome contains many replicons Table 14. 1 Eukaryotic replicons are small and I replicate more slowly than bacterial DNA Organism Replicons Lengt gth Movement Bacterium 4200kb50,000bp/min Yeast 500 40 kb 3, 600 bp/min Fruit fly 3.500 40 kb 2, 600 bp/min Toad 15000 200k 500 bp/ min Mouse 25000 150 kb 2, 200 bp/min Plant 35000 300kb 消当
12.4 Each eukaryotic chromosome contains many replicons