12.2 Origins can be mapped by autoradiography and electrophoresis UNIDIRECTIONAL REPLICATION Figure 12.5 Different densities of radioactive BIDIRECTIONAL REPLICATION labeling can be used to distinguish unidirectional and bidirectional Heavy density label Light density label replication Not labeled (invisible on autoradiograph) 消当
Figure 12.5 Different densities of radioactive labeling can be used to distinguish unidirectional and bidirectional replication. 12.2 Origins can be mapped by autoradiography and electrophoresis
12.2 Origins can be mapped by autoradiography and electrophoresis Simple Y Double Y Bubble Asymmetric bubble Figure 12.6 The position of the origin and the Fragment size doubles during number of replicating replication <><< forks determine the shape of a replicating restriction fragment, which can be followed by its Second dimension electrophoretic path(solid exaggerates contribution line). The dashed line of 3D shape 2 kb 2kb、12kt- shows the path for a linear 1 kb 1 kb 1 kb 1 kb DNA First dimension separates by 消当
Figure 12.6 The position of the origin and the number of replicating forks determine the shape of a replicating restriction fragment, which can be followed by its electrophoretic path (solid line). The dashed line shows the path for a linear DNA. 12.2 Origins can be mapped by autoradiography and electrophoresis
Forks 12.3 The bacterial genome is a single circular replicon 444▲▲ terE. DA terr. B terminate fork teminate fork Figure 12.7 Replication termini in e. coli are located beyond the point at which the replication forks actually meet 消当 Replication fork 2 Origin Replication fork 1
Figure 12.7 Replication termini in E. coli are located beyond the point at which the replication forks actually meet. 12.3 The bacterial genome is a single circular replicon
Forks 12.3 The bacterial genome is a single circular replicon 444▲▲ terE. DA terr. B terminate fork teminate fork Figure 12.7 Replication termini in e. coli are located beyond the point at which the replication forks actually meet 消当 Replication fork 2 Origin Replication fork 1
Figure 12.7 Replication termini in E. coli are located beyond the point at which the replication forks actually meet. 12.3 The bacterial genome is a single circular replicon
12. 4 Each eukaryotic chromosome contains many replicons S phase is the restricted part of the eukaryotic cell cycle during which 令 synthesis of DNA occur
S phase is the restricted part of the eukaryotic cell cycle during which synthesis of DNA occurs. 12.4 Each eukaryotic chromosome contains many replicons