DNA replication Pre-replicative complex formation and transition to replication Overall direction Topoisomerase I 5.99.12]29 of replication Purine Metabolism Topoisomerasell145 Topoisomerase lll2 Pyrimidine Metabollsm Genes coding for enzymes/proteins involved in DNA replication 1Helicase Single-stranded DNA primase24 Binding proteins 25 Replication factor-C 2Sliding clamp DNA polymerase (Pol8)2777 07DNA polymerase (Pola) 5 Sliding clamp2 27DNA polymerase (Pols/Pol) RNA primer Nucleotide excision repair 6511 DNA ligase 38 5 3 Lagging strand with Okazaki fragments Leading strand
OVERALL TOPOISOMERASES DIRECTION OF REPLICATION HELICASE SINGLE-STRAND BINDING PROTEINS DNA DNA POLYMERASE rNa PRIMASE Replication DNA POLYMERASE RNA ONA LIGASE PRIMER LAGGING STRAND LEADING WITH OKAZAK灯 STRAND FRAGMENTS
DNA Replication
Leading strand template S多H Leading strand ○kazaki Lagging fragment 8 strand Parent DNA Lagging strand template e 2001 Sinauer Associates,Inc (三)基因与性状遗传 碱基顺序 基因(DNA的片断)碱基顺序 基因氨基酸 性状 ①性状的遗传 ②遗传信息的转达 ③即亲代基因的子代表现 ④碱基顺序的转录过程（复制过程)
（三）基因与性状遗传 碱基顺序——基因（DNA的片断）碱基顺序 基因 氨基酸 性状 ①性状的遗传 ②遗传信息的转达 ③ 即亲代基因的子代表现 ④ 碱基顺序的转录过程（复制过程）
e-■-e wild-type fas1 Figure.Reverse genetics.Top,gene structure of FASCIATA 1 with exons in blue and introns in yellow.Red denotes a bacterial T-DNA that has inserted into the gene.Bottom,mutants lacking the wild- type FASI gene have altered development
Figure . Reverse genetics. Top, gene structure of FASCIATA 1 with exons in blue and introns in yellow. Red denotes a bacterial T-DNA that has inserted into the gene. Bottom, mutants lacking the wildtype FAS1 gene have altered development