Primer designed with the help of computer dna database conservative region comparision primers or blast
Primer designed with the help of computer DNA database conservative region comparision primers or blast
PCR reaction components and their functions template: SSDNA, dsDNA mrna needed to be rt as cdna samples: from blood, sperm or any other tissue from older forensic specimens from ancient biological samples or in the lab From bacterial colonies or phage plaques DNA: very stable
PCR reaction components and their functions template：ssDNA, dsDNA mRNA needed to be RT as cDNA samples：from blood,sperm or any other tissue,from older forensic specimens, from ancient biological samples or in the lab. From bacterial colonies or phage plaques. DNA：very stable
primes If the pcr product is to be cloned it is sensible to include the sequence of unique restriction enzyme sites within the 5-ends of the primers Can amplify fragments over 10kb in length Store:纯化引物在25%乙腈溶液中4°C; 冻千引物于-20°C可保存1-2年,液体状 态于-20°C可保存6个月。不用时应-20°C 保存
primes If the PCR product is to be cloned, it is sensible to include the sequence of unique restriction enzyme sites within the 5’-ends of the primers. Can amplify fragments over 10kb in length Store:纯化引物在25％乙腈溶液中4℃； 冻干引物于-20℃可保存1-2年，液体状 态于-20℃可保存6个月。不用时应-20℃ 保存
buffer 10-50 mmol/L Tris-HC|pH83-88,20°C)。 Mg+: for Taq polymerase activity conc. Low: low activity high: non-specific amplification dNTPs: 贮备dNTP液:1mo/ L NaOh调pH至中性。 贮备浓度为5-10mmol/L,终浓度为20-200umo/L, 分装-20°C保存。 4种dNTP浓度应相同
buffer 10-50mmol/L Tris-HCl(pH8.3-8.8, 20℃)。 Mg2+：for Taq polymerase activity conc.:Low:low activity high: non-specific amplification dNTPs： 贮备dNTP液：1 mol/L NaOH 调pH至中性。 贮备浓度为5-10mmol/L，终浓度为20-200umol/L， 分装-20℃保存。 4种dNTP浓度应相同
Taq dNa polymerase: from thermophilic bacteria Taq polymerase from Thermus aquaticus other thermostable dna polymerase with greater accuracy used for certain applications mineral oil
Taq DNA polymerase： from thermophilic bacteria. Taq polymerase from Thermus aquaticus. other thermostable DNA polymerase with greater accuracy used for certain applications. mineral oil