Report vector Synthetic poly (A) signal /transcriptional pause site (for background Amp reduction) f1 Mlul 15 Nhel 21 Smal 28 pGL3-Basic Xhol 3 Vector Balll 36 (4818bp) HindIll 53 Ncol 86 2010 sall 2004 BamHI JuC+ Narl 121 SV40 late poly (A)signal (for luc+ reporter) Hpal 1902 Xbal 1742 Department of Biochemistry Molecular Biology e 16
Department of Biochemistry & Molecular Biology 16 Report Vector
Report vector Synthetic poly(A) signal/transcriptional pause site for background Amp reduction) Kong f1 or Sacl[11 Nhel Smal 28 pGL3-Promoter Xhol 32 Vector BgⅢ36 (5010bp) 23 Sall SV40 Promoter BamHI SV40 late poly (A) signal dl245 for /uc+ reporter) /uC+ Ncol 278 Hpal 2094 Xbal 1934 Department of Biochemistry Molecular Biology e
Department of Biochemistry & Molecular Biology 17 Report Vector
Report vector Synthetic poly(A) signal/transcriptional ause site (for background Ampr reduction Sacl11 f1 ori Mu|15 Nhel 21 Smal 28 pGL3-Enhancer Bg|36 Vector Hindlll 53 (5064bp) 225a11 l86 Narl 121 2250 BamHI S√40 Enhancer SV40 late poly(A)signal (for luc+ reporter) Hpal 1902 Xbal 1742 Department of Biochemistry Molecular Biology e 18
Department of Biochemistry & Molecular Biology 18 Report Vector
Report vector Synthetic poly(A) signal / transcriptional pause site (for background Am reduction) Kpnl 5 f1 ori Sac Mu|15 Nhel 21 gl3-Control Smal28 Xhol Vector BgⅢl 2448 Sall 2442 BamHI (5256b SV40 Promoter d245 SV40 Enhancer luC+ Ncol 278 Narl 313 SV40 late poly(A)signal (for luc+ reporter Hpal 2094 Xbal 1934 Department of Biochemistry Molecular Biology e 19
Department of Biochemistry & Molecular Biology 19 Report Vector
or backaround Amp' reduction f1 ori pGL3-Basic 1528263 pGL3-Promoter 4818bp) (5010bp) SV40 Promoter 2196 BamHI SV40 late Narl 121 poly(A) signal HindIll 24 Ncol 278 Hpal 2094 Sv4o late (for luc+ reporte Hpal 1902 Xbal 1742 Xbal 193 Synthetic poly(A) Synthetic poly(A) signal/transcrip signal /transcriptional for background ( for background reduction) reduction f1 ori f1 ori Mlul GL3-Enhancer Vector pGL3-Control Xhol Vector 2448 YNcol 86 (5256bp) 2256| SV40 Promoter 2250 BamH luC+ Hindlll 245 SV40 Enhance SV40 Enhancer Ncol 278 UC+ Narl 313 SV40 late SV40 late oly(A) signal (for luc+ reporter (for luc+ reporter) Xbal 1742 Hpal 2094 Xbal 1934
Department of Biochemistry & Molecular Biology 20