A NOTEr It is important to appreciate that when we talk about isolating and purifying a given macromolecule in the ensuing discussion we rarely mean that a single molecule is isolated. rather the goal of these procedures is to isolate a large population of identical molecules from all of the other kinds of molecules in cell
A NOTE: It is important to appreciate that when we talk about isolating and purifying a given macromolecule in the ensuing discussion we rarely mean that a single molecule is isolated.rather,the goal of these procedures is to isolate a large population of identical molecules from all of the other kinds of molecules in cell
NUCLEIC ACIDS
NUCLEIC ACIDS
1.1 Electrophoresis through a Gel separates DNA and RNA Molecules According to Size This part is devoted to techniques for the manipulation and characterization of nucleic acids from the isolation of rnas and DNAs to the sequencing of entire genomes and comparative genomICs
This part is devoted to techniques for the manipulation and characterization of nucleic acids,from the isolation of RNAs and DNAs to the sequencing of entire genomes and comparative genomics. 1.1 Electrophoresis through a Gel separates DNA and RNA Molecules According to Size
Gel electrophoresis(凝胶电泳) separation of dna and rna molecules. Linear dna molecules separate according to size when subject to an electric field through a gel matrix(an inert jello-like porous material)动画
Gel electrophoresis (凝胶电泳) separation of DNA and RNA molecules.Linear DNA molecules separate according to size when subject to an electric field through a gel matrix(an inert,jello-like porous material)动画
DNA molecules are flexible and occupy an effective volume pores in the gel matrix sieve the dna molecules according to this volume; large molecules migrate more slowly through the gel because they have a larger effective volume than do smaller dNas and thus have more difficulty passing through the interstices of the gel Different sizes are separated because they moved different distances through the gel
DNA molecules are flexible and occupy an effective volume pores in the gel matrix sieve the DNA molecules according to this volume;large molecules migrate more slowly through the gel because they have a larger effective volume than do smaller DNAs,and thus have more difficulty passing through the interstices of the gel. Different sizes are separated because they moved different distances through the gel