Table 471. Gram and acidfast stainingmethods.Gram stain(1) Fixsmearby heat(2) Coverwith crystal violet(3)WashwithwaterDnotbo(4)CoverwithGramsiodine(5)Washwithwateotblot.(6)Decolorizefor1030secondswithgentleagitationinacetone(30 mL) and alcohol (70 mL)(2) Wash with waterDo not blot.(8)overfor130secondswithsafranin2.5%solutionin95% alcohol.(9)WashwithwaterandletdryZiehl-Neelsenacid-fast stain(1) Fixsmearby heat(2witbfumuoverdirectflame（orfor20minutesoverawaterbath(3) Wash withwater.(4)Decoorizeinacidalcoholuntilonlyafaintpinkcooremains.(5) Wash with water.(6) Counterstinfor1030 secondswithLoeflersmethylene blue.(7) Wash with waterand letdryKinyoun carbolfuchsin acid-faststair(omulbasifuchinph20m95%achol,100 m disile water(2)Stainfixedsmearfor3minutes(noheatecearyandcontinue as withZieh-Neelsen stainSHIHEZI UNIVERSITY
SHIHEZI UNIVERSITY
SHIHEZI UNIVERSITY
SHIHEZI UNIVERSITY
WNI一中中AWSHIHEZI UNIVERSITY
SHIHEZI UNIVERSITY
SHIHEZI UNIVERSITY
SHIHEZI UNIVERSITY