Example: human IL-17B lL-17E(-25) l17B1u-17ct-17D IL-17RA IL-17RC IL-17RB IL-17RE SEFIR NF-KB CEBP AP-1(NF-KB(CEBP Figure 1. IL-17 and the IL-17 Receptor Families Immunity.201134(2):149-162 Chang, S.H., Reynolds, J.M. Pappu, B P. Chen, G, Martinez, G.J., and Dong, C. Interleukin-17C promotes Th17 cell responses and autoimmune disease via interleukin-17 receptor E Immunity, 201 35,611-621. Reynolds, J.M., Martinez, G.J., Nallaparaju, K.C., Chang, S.H., Wang, Y.H., and Dong, C. Cuting edge regulation of intestinal inflammation and barrier function by IL-17C. J Immunol, 2012, 1894226-4230
微生物与生化药学教研室 Immunity. 2011 34(2):149-162 IL-17RE Chang, S.H., Reynolds, J.M., Pappu, B.P., Chen, G., Martinez, G.J., and Dong, C. Interleukin-17C promotes Th17 cell responses and autoimmune disease via interleukin-17 receptor E. Immunity, 2011, 35, 611-621. Reynolds, J.M., Martinez, G.J., Nallaparaju, K.C., Chang, S.H., Wang, Y.H., and Dong, C. Cutting edge: regulation of intestinal inflammation and barrier function by IL-17C. J Immunol, 2012,189, 4226-4230
Example: human IL-17B Immunity Cell Volume42 Issue d, 21 April 2015, Pages 692-703 Interleukin-17B Antagonizes Interleukin-25-Mediated Mucosal Inflammation Joseph M. Reynolds 1,7. Young-Hee Lee2,7. Yun shi xiaohu wang. Pornpimon Angkasekwinai Kalyan C. Nallaparaju2, stephanie Flaherty 1, Seon Hee Chang, Hiroshi Watarai. 6, Chen Donga, a Received 20 August 2014. Revised December 2014 Accepted 20 March 2015. Available online 14 April 2015 Published: April 14,201 Oncogene.2014Jun533(23)2968-77.dot.10.1038nc2013268.Epu2013Ju15 Autocrine/paracrine mechanism of interleukin-17B receptor promotes breast tumorigenesis through NF-kB mediated antiapoptotic pathway. Huang CK1 Yang CY2, Jeng YM3, Chen CL2 Wu HH2, Chang YC2 Ma C2, Kuo WHf, Chang kj5 Shew JY1, Lee WH6
微生物与生化药学教研室
Role of il-17b in the colon cancer human IL-17B expression and IL-17RB expression in colon cancer cell line and colon cancer biospy IL-17b IL-17b overexpressed knockdown colon WT colon cells cells (PCDNA3. shRNA) In vitro In vivo (xenograft model) cell proliferation apoptosis Tumor growth survival proliferation marker colon formation(2D and 3D) apoptosis marker
微生物与生化药学教研室 human IL-17B expression and IL-17RB expression in colon cancer cell line and colon cancer biospy IL-17b knockdown colon cells (shRNA) WT In vivo （xenograft model) IL-17b overexpressed colon cells (pCDNA3.1) In vitro cell proliferation apoptosis survival colon formation (2D and 3D) Tumor growth proliferation marker apoptosis marker
How to get the target gene sequence? http://www.ncbinim.nihgov Display settings: Summary, 20 per page, sorted by Detain snd1D:⊙Fen: Manage Eite Anmah(/0 Results: 1 to 20 of 3807 p1e,La… Results by tason U Found 11500 ncode seqences Nuckotde (aon) EST (SB3)GSs(10) Top organisms [reel Vibro cholerae (To/ a The following term were not found in Nuco humant,humar.17B Aeromona Mycobacterium tuberculosis(91) 1. 222. 006 bp linear DNA Aeeesnon NC o/8811 o inyo 图A(w2 U Aeromonas phage. Px29 compete genome 2 222 006 bp linear DNA Find related data 0100103122424 DatabaseSelect Homo nap eos interleukin 178 UL17B)mRNA search details U Mus musculus A kinase IPRKA anchor proteln 178 LAkap17D) mRNA Pann c1 4 6. 153 bp lnear mRNA Neeson hM nninaineI n IMh
微生物与生化药学教研室 http://www.ncbi.nlm.nih.gov How to get the target gene sequence?
hIL- 17B gene T Atggactggcctcacaacctgctgtttcttcttaccatttccatcttcctggggctgggccagcccag gage cccaaaagcaagaggaaggggcaagggcggcctgggcccctggcccctggccctcaccaggtgccac tggacctggtgtcacggatgaaaccgtatg cccgcatggaggagtatgagaggaacatcgagga gat ggtggcccagctgaggaa cagctcagagctg gcccagagaaagtgtgaggtcaacttgcagctgtgg atgtccaacaagaggagcctgtctccctggggctacagcatcaaccacgaccccagccgtatccccgtg gacctgccggaggcacggtgcctgtgtctgggctgtgtgaaccccttcaccatgcaggaggaccgca gc atggtgagcgtgccggtgttcagccaggttcctgtgcgccgccgcctctgcccgccaccgccccgcacag ggccttgccgccagcgcgcagtcatg gagaccatcgctgtgggctgcacctgcatcttctga
微生物与生化药学教研室 Atggactggcctcacaacctgctgtttcttcttaccatttccatcttcctggggctgggccagcccaggagc cccaaaagcaagaggaaggggcaagggcggcctgggcccctggcccctggccctcaccaggtgccac tggacctggtgtcacggatgaaaccgtatgcccgcatggaggagtatgagaggaacatcgaggagat ggtggcccagctgaggaacagctcagagctggcccagagaaagtgtgaggtcaacttgcagctgtgg atgtccaacaagaggagcctgtctccctggggctacagcatcaaccacgaccccagccgtatccccgtg gacctgccggaggcacggtgcctgtgtctgggctgtgtgaaccccttcaccatgcaggaggaccgcagc atggtgagcgtgccggtgttcagccaggttcctgtgcgccgccgcctctgcccgccaccgccccgcacag ggccttgccgccagcgcgcagtcatggagaccatcgctgtgggctgcacctgcatcttctga hIL-17B gene