A. Molecular basis of oxidation: Electron transport chain Intermembrane space Ⅳ Matrix cH' Succinate NADH IFADHJ 1/202+2 Complex I Complex Il Complex I Complex IV H Cytochrome bcl Succinate Cytochrome c hydrogenase dehydrogenase Oxidase SUBUNITS Mammalian mtDNA 3 nDNA 35 4 10 TOTAL 11 13
A. Molecular basis of oxidation: Electrontransport chain
B. Molecular basis of phosphorylation: ATP synthase The structure of the ATP synthase As- FI particle is the catalytic subunit; The Fo particle attaches to FI and is embedded in the inner membrane F: 5 subunits in s the ratio 3a:3β:1y:l8:l8 F.1a:2b:12c E Matrix 00m ccclclclcl intermembrane space GUU
B. Molecular basis of phosphorylation: ATP synthase ❖ The structure of the ATP synthase F1 particle is the catalytic subunit; The F0 particle attaches to F1 and is embedded in the inner membrane. F1 : 5 subunits in the ratio 3:3:1:1:1 F0: 1a：2b：12c
FI particles have ATP synthase activity nner mitochondia (b) Submitochondrial partc es Electron transport? Yes sTP synthess? Yes Outer mitochondrial ATPase activity? No membrare (a) Intact mitochondrion Electron transport? Yes ATP Synthesis? Yos Eectron transpor? Ye ATP synthesis? No ○ ATPase activty? Yes : (d) Membranous fractio (e)Soluble fraction wth F, spheres Eectron transport? Yes Electron transport? No ATP Synthesis? N ATP synthesis? No ATPase activity? No ATPase activity? Yes (f) Reconstitutea partcles Electron transport? Yes ATP synthesis? Yos 1No
❖F1 particles have ATP synthase activity
o Proton translocation through Fo drives ATP synthesis by F: Binding change model and rotational catalysis @Cycle begins again Loose subunit with knob rotate binds aDP + P Boyer proposed in by one subunit ADP 1979 and was F knob, top view greatly stimulated / by the publication ADP+p in 1994 of the structure for F1 6° rotation complex(x-ray) from bovine heart mitochondria (New ATP forms by ② Energy input alters condensation; ongin ATP is released: L subunit ailable for bindir
❖ Proton translocation through F0 drives ATP synthesis by F1 : Binding Change Model and rotational catalysis Boyer proposed in 1979, and was greatly stimulated by the publication in 1994 of the structure for F1 complex (X-ray) from bovine heart mitochondria
o Direct experimental evidence supporting the rotational catalysis Actin filament Japan researcher a3B3r B Nature 386: 300 complex 1997 His-tag Coverslip coated with N-NTA
❖Direct experimental evidence supporting the rotational catalysis. Japan researcher, Nature 386: 300, 1997