细菌培养 平皿分高和摇瓶培养
细菌培养 平皿分离和摇瓶培养
细菌的基因组 One circular chromosome 4-5Mb Parent cell Cell wall Chromosome Origin of replication Nucleoid Replication fork Daughter cells
细菌的基因组 One circular chromosome 4-5Mb
质粒 (产 (b) Tn5 n3 IS1 Sm IS1 Tn4 \+-Resistance-determinant segment S2
质粒
二、大肠杆菌遗传 Escherichia coli. Scanning electron micrograph of E.coi(×14000)
二、大肠杆菌遗传
EcOl染色体的结构 E coli 4.6Mb. 4288genes E coli K12 DNA sequence, 1997 90% encode protein 8 locations of remnants of bacteriophage Y genomes Insertion sequences(s)
E.coli 染色体的结构 • E.coli 4.6Mb. 4288genes • E.coli K12 DNA sequence，1997 • 90% encode protein • 8 locations of remnants of bacteriophage genomes. • Insertion sequences (IS)