目的基因 EGFP(720bp)的基因片段 GGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCO GACGGO AGG ATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTO GGAGTTCGTGA 目的基因内限制性酶切位点如下 154 ECo57I 198 Eco57 397 Eco57I Bsp1407181 Bail
目的基因： EGFP (720bp)的基因片段: ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGA CGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGA CCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGA CCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCG CCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCC GCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTC AAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATC ATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGG CAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCC CGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACA TGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA 目的基因内限制性酶切位点如下： 154 Eco57I 198 Eco57I 397 Eco57I 710 Bsp1407I 181 BsiI
序列来源: pEGFP-M3质粒 Asel SnaB I ApaLI MCS pUC CMV IE Ec0109 Sy TK EGFP 则 pEGFP-N3 4.7kb BsrG I Syn Kan/ Neo Sy40 on f1 A∥l a Stul EGFP GCTAGC GCT ACC GGA CTC AGA TCT CGA GCT CAA GCT TCG AAT TCT GCA GTC GAC GGT ACC GCG GGC CCG GGA TCC ATC GCC ACC ATG GTG Nhe l Eco47 Ill 6g‖ Xho l Saci Hind Ill EcoR I PstI Sall Kpal Apal BamH ‰ml Ec/36ll AccI Asp7181\.6spl201 Xmal Smal
序列来源：pEGFP-N3质粒