In vitro assessment can be performed in intact cell lines or cell-free systems(非细胞系统). Intact cell lines:For example,some new drugs for tumors. The effects of the new drugs on tumor cell growth,cell cycle distribution,cell differentiation,or the induction of apoptosis can also be readily measured. Breast cancer cell lines 2019/8/28 Institute of clinical pharmacology,AHMU 11
In vitro assessment can be performed in intact cell lines or cell-free systems(非细胞系统）. Intact cell lines: For example, some new drugs for tumors. The effects of the new drugs on tumor cell growth, cell cycle distribution, cell differentiation, or the induction of 2019/8/28 Institute of clinical pharmacology, AHMU 11 apoptosis can also be readily measured. Breast cancer cell lines
60 human tumor cell lines were used by the NCI (National Cancer Institute,USA as the principle anticancer screening instrument to identify the growth inhibitory activity against human tumor cell lines of new drug. Hela (Cervical cancer) HT29 (Colon cancer BaF3 (B cell lines of mice) 2019/8/28 Institute of clinical pharmacology,AHMU 12
60 human tumor cell lines were used by the NCI (National Cancer Institute, USA ) as the principle anticancer screening instrument to identify the growth inhibitory activity against human tumor cell lines of new drug. 2019/8/28 Institute of clinical pharmacology, AHMU 12 Hela (Cervical cancer ) HT29 (Colon cancer ) BaF3 (B cell lines of mice )
Cell-free systems assays include to measure: 1)enzyme inhibition, 2)receptor binding, 3)protein-small molecule interactions, 4)interference with components of important signal transduction pathways. 2019/8/28 Institute of clinical pharmacology,AHMU 13
Cell-free systems assays include to measure： 1) enzyme inhibition, 2) receptor binding, 3) protein-small molecule interactions, 4) interference with components of important signal 2019/8/28 Institute of clinical pharmacology, AHMU 13 transduction pathways
Some techniques,such as nuclear magnetic resonance (NMR)and X-ray crystallography in cell-free systems,also can be vital in gathering important information for the design of specifically targeted drug therapies. ACHN MD simuiation TF.Tbr STD NMR Poe】 Pose 2 NMR X-ray crystallography 2019/8/28 Institute of clinical pharmacology,AHMU 14
• Some techniques, such as nuclear magnetic resonance (NMR) and X-ray crystallography in cell-free systems, also can be vital in gathering important information for the design of specifically targeted drug therapies. 2019/8/28 Institute of clinical pharmacology, AHMU 14 NMR X-ray crystallography
Another approach to preclinical in vitro drug testing is to use genetically characterized cell lines to assess drug effects against cells containing known specific DNA mutations. For example,NCI has been studying the use of genetically defined yeast strains as a screening tool for the testing of potential anticancer agents in vitro. 2019/8/28 Institute of clinical pharmacology,AHMU 15
• Another approach to preclinical in vitro drug testing is to use genetically characterized cell lines to assess drug effects against cells containing known specific DNA mutations. • For example, NCI has been studying the use of genetically defined yeast strains as a screening tool for the testing of potential anticancer agents in vitro. 2019/8/28 Institute of clinical pharmacology, AHMU 15