Strategies for controlling of GCG 了防治方法：农业防治、物理防治、化学防治 抗病品种（遗传工程）、生物防治 生防菌株：F2/5、MI15、E26
Strategies for controlling of GCG 防治方法：农业防治、物理防治、化学防治 抗病品种（遗传工程）、生物防治 生防菌株：F2/5、MI15、E26
Agrobacterium vitis E26 Isolation:originally isolated from vineyard; Identified as A.vitis according to: Morphology Physiological and Biochemistrical tests 16SrDNA analysis Pathogencity tests:nonpathogenic on grape, sunflower and tomato,etc. Production of antibiotics:producing agrocin active against pathogenic A.vitis in vitro
Agrobacterium vitis E26 • Isolation: originally isolated from vineyard; • Identified as A. vitis according to: - Morphology - Physiological and Biochemistrical tests - 16SrDNA analysis • Pathogencity tests: nonpathogenic on grape, sunflower and tomato, etc. • Production of antibiotics: producing agrocin active against pathogenic A. vitis in vitro
12 菌剂加入等体积的水混匀根部浸泡20至30分钟 324 蘸根处理葡萄苗 经处理的葡萄苗未经处理的葡萄苗
经处理的葡萄苗 未经处理的葡萄苗
A.tumefaciens (Ti plasmid) as genes vectors 口A natural genetic engineer(天然的基因工程师) Strategy -add eukaryotic promoter(真核细胞基因的启动子)to desirable gene(目的基因) eg insecticidal protein of B.thuringiensis insert desirable gene into T-DNA,in Ti plasmid insert plant T-DNA inserts into plant genome-expresses gene
A. tumefaciens (Ti plasmid) as genes vectors A natural genetic engineer（天然的基因工程师） Strategy - add eukaryotic promoter（真核细胞基因的启动子） to desirable gene（目的基因） + eg insecticidal protein of B. thuringiensis - insert desirable gene into T-DNA, in Ti plasmid - insert plant → T-DNA inserts into plant genome → expresses gene
2.Erwinia(欧文氏菌属) straight rod-shaped(直杆状) Peritrichous flagella (most)(大多数周生多鞭毛) 6- facultative saprophyte(兼性腐生) Diseases caused Soft rot(软腐)，bacterial wilt（萎蔫)，fire blight(火疫) Symptom rot,leaf spot,wilt
2. Erwinia (欧文氏菌属) • straight rod-shaped(直杆状） • Peritrichous flagella (most)（大多数周生多鞭毛） • G- • facultative saprophyte（兼性腐生） • Diseases caused Soft rot（软腐）, bacterial wilt（萎蔫）, fire blight（火疫） • Symptom rot, leaf spot, wilt