19.3 DNA is coiled in arrays of nucleosomes 受⌒ 200 bp 185bi 146bp Mono Trimmed Core particles nucleosomes nucle oso mes Figure 19.10 Microccocal nuclease initially cleaves between nucleosomes Mononucleosomes typically have 200 bp dna End-trimming reduces the length of DNa first to 165 bp, and then generates core particles with 146 bp 消当
Figure 19.10 Microccocal nuclease initially cleaves between nucleosomes. Mononucleosomes typically have ~200 bp DNA. End-trimming reduces the length of DNA first to ~165 bp, and then generates core particles with 146 bp. 19.3 DNA is coiled in arrays of nucleosomes
19.3 DNA is coiled in arrays of nucleosomes DNA"leaves Figure 19.4 The nucleosome may be a cylinder with DNA organize d DNA"enters" Into two turns around the surface 消当
Figure 19.4 The nucleosome may be a cylinder with DNA organized into two turns around the surface. 19.3 DNA is coiled in arrays of nucleosomes
19. 4 DNA Structure varies on the nucleosomal surface MM Denature Labeled fragm ent mnmmamaagmmanRRRRERRIE Elec rophoresis Figure 19 11 Nicks in double-stranded dna are revealed by fragments when the DNA is denatured to give single strands. If the dna is labeled at( say)5 ends, only the 5 fragments are visible by autoradiography. The size of the fragment dentifies the distance of the nick from the labeled end 笨大
Figure 19.11 Nicks in double-stranded DNA are revealed by fragments when the DNA is denatured to give single strands. If the DNA is labeled at (say) 5 ends, only the 5 fragments are visible by autoradiography. The size of the fragment identifies the distance of the nick from the labeled end. 19.4 DNA structure varies on the nucleosomal surface
19. 4 DNA Structure varies on the nucleosomal surface Figure 2.4 When GLFEDBLA <Restriction mal restriction fragments are identified by their Fragments identified on gel possession of a labeled 口 A-G(unbroken end. each fragment A-日-C-D-EF directly shows the 日自EBE distance of a cutting site A-日-C from the end A-B Successive fragments increase in length by the distance between Labeled end adjacent restriction sites 消当
Figure 2.4 When restriction fragments are identified by their possession of a labeled end, each fragment directly shows the distance of a cutting site from the end. Successive fragments increase in length by the distance between adjacent restriction sites. 19.4 DNA structure varies on the nucleosomal surface
19. 4 DNA Structure varies on the nucleosomal surface 59 58 57 56 Figure 19 12 Sites for nicking lie at 55 regular intervals along core DNA,as 54 seen in a DNAase I digest of nuclei Photograph kindly provided by Leonard lutter 消当
Figure 19.12 Sites for nicking lie at regular intervals along core DNA, as seen in a DNAase I digest of nuclei. Photograph kindly provided by Leonard Lutter. 19.4 DNA structure varies on the nucleosomal surface