Figure21-1.Gramstainofaurethralexudateofapatientwithgonorhea.Nucleiandcellmembraneoutinesofmanypolymorphonuclearcellsareseen(twshownbylargearows).lntracellulargram-negativediplococci (Neisseria gonorrhoeae) in clumps aremarked by thesmall arrows.SHIHEZIUNIVERSITY
SHIHEZI UNIVERSITY
Neisseragonorrhoeae-coccoidprokarye divdingcausesgonha(SEMx40000)DrDennisKunkel,UniversityofHawaiUsed with permissionSHIHEZIUNIVERSITY
Neisseria gonorrhoeae - coccoid prokaryote (dividing); causes gonorrhea (SEM x 40,000) © Dr Dennis Kunkel, University of Hawaii. Used with permission SHIHEZI UNIVERSITY
CultivationCultureObligatory aerobicThese bacteria grow best on chocolate agar so-calledbecause it contains heated blood brown in color Thayer Martin Chocolate Agar A modified (selective) chocolate agar Biological reactions+Glucose fermentation onlyCan not use other sugarsSHIHEZI UNIVERSITY
Cultivation Culture Obligatory aerobic These bacteria grow best on chocolate agar so-called because it contains heated blood brown in color Thayer Martin Chocolate Agar A modified (selective) chocolate agar Biological reactions Glucose fermentation only Can not use other sugars SHIHEZI UNIVERSITY
Oxidase Test The colonies areoxidase positivewhich isdemonstrated byflooding the platewith a dye which onoxidation changescolorPhoto by Karen M.KiscrSHIHEZI UNIVERSITY
Oxidase Test The colonies are oxidase positive which is demonstrated by flooding the plate with a dye which on oxidation changes color SHIHEZI UNIVERSITY
Surface Antigenic StructuresOutermembranePeptidoglycanCell envelopeCytoplasmicPeptidoglycanmembraneOutermembraneCytoplasmicmembranePilusPilusFigure21-2.CollageanddrawingofNgonorrhoeaeshowing piliand the threelayers ofthe cellenvelopeSHIHEZIUNIVERSITY
Surface Antigenic Structures SHIHEZI UNIVERSITY