二、基因诊断的常用技术与方法 (一)基因诊断的常用技术 (二)基因诊断的基本方法
二、基因诊断的常用技术与方法 (一) 基因诊断的常用技术 (二) 基因诊断的基本方法
(一)基因诊断常用技术 1.核酸分子杂交 2.PCR(聚合酶链式反应) 3.限制性酶切分析 4.SSCP(单链构象多态性分析) 5.DNA测序 6.DNA芯片技术
(一)基因诊断常用技术 1. 核酸分子杂交 2. PCR（聚合酶链式反应） 3. 限制性酶切分析 4. SSCP（单链构象多态性分析） 5. DNA 测序 6. DNA 芯片技术
基因诊断技术分类 Molecular hybridization Southern,Northern,slot blot,FISH,ASO microarray ·PCR PCR/ASO,PCR/SSCP,PCR/sequencing.PCR/RFLP
基因诊断技术分类 • Molecular hybridization Southern, Northern, slot blot, FISH, ASO, microarray • PCR • PCR/ASO, PCR/SSCP, PCR/sequencing, PCR/RFLP
Targets and Molecular Techniques DNA Southern blot,FISH,PCR,Sequencing,microarray restriction analysis,RFLP,SSCP RNA Northern blot,RT-PCR,microarrays Protein Western blot,Immuno-histochemistry, microarray
Targets and Molecular Techniques • DNA Southern blot, FISH,PCR, Sequencing, microarray, restriction analysis, RFLP，SSCP • RNA Northern blot, RT-PCR, microarrays • Protein Western blot, Immuno-histochemistry, microarray
Basic Principle DNA:denaturation and hybridization of the helix structure Southern,Fish PCR Protein:Antigen-antibody interaction )=→（●
Basic Principle Denature Hybridize Southern, Fish PCR • Protein: Antigen-antibody interaction ⚫ DNA: denaturation and hybridization of the helix structure