The presence of multiple antibiotic resistance is due to the fact that a single r plasmid contains a variety of genes coding for different antibiotic inactivation enzymes I H H.NC-N NH Biochemical HⅡ N-CNH2 HO mechanism OH RNH of resistance CH3 CH, mediated by OH R plasmids CHO Streptomycin Hc一cHcL H N H 3 O,N HH OH Phosphorylation (In aminoglycoside antibiotics Adenylylation with free amino group, inactivation also by N-acetylation
The presence of multiple antibiotic resistance is due to the fact that a single R plasmid contains a variety of genes coding for different antibiotic inactiviation enzymes Biochemical mechanism of resistance mediated by R plasmids
BamHI site Engineered plasmids-Artificial plasmids BamHI Foreign DNA · Incorporation BamHI into artificial plasmids of genes from a wide variety of DNA ligase sources has made possible the transfer of Hybrid containing Recycled foreign DNA genetic DBR322 material across Transformation of E col virtually any Transformants Transformants species barrier resistant to both resistant to ampicillin ampicillin and tetracycline but sensitive to tetracycline
Engineered plasmids-Artificial plasmids • Incorporation into artificial plasmids of genes from a wide variety of sources has made possible the transfer of genetic material across virtually any species barrier