Positive vs negative control Mutate Regulatory EXample of regulatory protein is regulatory gene to lose present protein function Positive control Operon ON Activator Operon OFF Negative control Operon OFF Repressor Operon ON
Positive vs negative control Regulatory protein is present Mutate regulatory gene to lose function Positive control Negative control Example of regulatory protein Operon ON Operon OFF Operon OFF Operon ON Activator Repressor
Catabolic vs. biosynthetic operons Operon encodes absence of effect presence of Effect Catabolic Induced Substrate Repressed Substrate enzymes (derepressed Biosynthetic Product Induced Product Repressed enzymes
Catabolic vs. biosynthetic operons Absence of Catabolic enzymes Biosynthetic enzymes Effect Induced Repressed Operon encodes Presence of Effect Substrate Product Product Substrate Induced (derepressed) Repressed