TableZ,DetectionoftheNovelCoronavirusinVariousClinicalSpecirmenswiththeUseofDifferentPCRAssays.*SampleResultonPCRAssayBNloutS2BNloutAsand NestedIN-2SARISBNloutS2BNlinSIN-4Real-TimePCRTSARIASBNloutAsBNlinAs++++Cell culturet8.3x106IndexpatientSputum,day91.0x10m-+++Throatswab,day9---+8005<8005Nasalswab,day9----Plasma,day9--+1901--+BAL,day11++4.1×10sStool, day 25NDNDNDND+Contact1-Sputum, day3+++6.3×104Throatswab,day3-----Nasalswab,day3------1Plasma,day3----Stool, day 19NDNDNDND+Contact2Sputum,day5------1Plasma,day5----NDNDNDND-Stool, day21*PCRdenotespolymerase chain reaction,BALbronchoalveolar-Javagefluid,and NDnotdone.Plus signs indicateposi-tiveresults,andminussigns negativeresults.f Values are copies permilliliter.Samplewastested24hoursafterpassage.Testwaspositivebutbelowthelimitofreliablequantification(soocopiespermilliliter).Testwasperformedafterultracentrifugationof2mlofplasma
有报道显示，在可能SARS病人中病毒的检出率为100%。在疑似SARS病人中的检出率为23%。所有健康接触者中未检测到病毒
◼ 有报道显示，在可能SARS病人中病毒的 检出率为100%。 ◼ 在疑似SARS病人中的检出率为23%。 ◼ 所有健康接触者中未检测到病毒
Table3.Proportionof PatientswithaPositiveRT-PCRResultforCoronavirus.Mean No.ofSamplesFraction of Patients TestingGroupPositiveperPatientIN-6/IN-7BNloutS2]andNestedBNloutAsSARIS/and NestedSARIAsBNlinS/BNlinAs5/52.25/5PatientswithprobableSARS1.33/133/13Patientswithsuspected SARSi1.00/210/21Contacts* RT-PCR denotes reverse-transcriptasepolymerase chain reaction.Sampleswerefromthelowerrespiratorytract in fivepatients andnasopharyngeal swabs in one patient (all positive);samples were obtained 3to 13 daysaftertheonsetofillness.Nasopharyngeal samplesfrom13patients wereused;theywereobtained3to12daysaftertheonsetofillness
另一项研究显示检测病毒的3种方法（血清学检测、病毒分离、PCR技术）中，PCR技术的检出率最高
另一项研究显示 检测病毒的3种方法（血清学检测、病 毒分离、PCR技术）中，PCR技术的检出率 最高
Tablel.SpecimensfromPatientswithSARsThatWerePositiveforSARS-AssociatedCoronavirusbyOneor.MoreMethods.wFindingsPatientHospital-SerologicExposureon ChestRT-PCRAge/SexRadiographSpecimenIsolationNo.and SettingIzationResults1Yes+一Singapore,53.yr/FPneumoniaNasal,oropharyngealNot donehospitalswabs24+Hong Kong.36yr/FPneumoniaYesNasal,swab-Not donehotei3Hong Kong.22yr/MYesXSwabPneumonia一-hotel4Yes+-Hong Kong.39yr/MPneumoniaNasal,pharyngeal-hotelswab5++HongKong.49yr/MPneumoniaYesNot doneSputumhotel+s6+HongKong.46yr/MYes+Kidney,lung.bron-+Pneumoniahotelchoalveolarlavage7Vietnam,Yes++Adult/PneumoniaOropharyngealwashhospitalunknown8Vietnam.AdultlYesOropharyngealwash一+Pneumonia-hospitalunknown9Vietnam,AdultlPneumoniaYesOropharyngealwash--+hospitalunknown10Vietnam,Adult/YesPneumoniaOropharyngealwash+--hospitalunknownX11YesVietnam.Adult/PneumoniaOropharyngealwash-hospitalunknown12+Vietnam.Adult/YesPneumoniaOropharyngealwash-一hospitalunknown13Vietnam.Adult!YesOropharyngealwash++Pneumonia-hospitalunknown14Yes+Vietnam,Adult/Pneumonia-Oropharyngealwash-hospitalunknown15Vietnam.AdultlYesOropharyngealwash+Pneumonla一-hospitalunknown+116Vietnam,46y//MYes+Nasal,oropharyngealPneumonia+hospitalswab17+Canada,43yr/MPneumoniaYesNot doneLung.bonemarrow-Famity18Taiwan,51yr/FPneumoniaYes+一Sputum一family19Hong Kong.Adult/FYes+PneumoniaOropharyngealwash-吉hotelwPlussignsdenotepositiveresults,andminus signs negativeresults,Theserologicand RT-PCRassayswerenotnecessarilyperformed onsamples obtainedatthesametime.Thiswaslatespecimen.antibodypositiveatfirstsample.TTravelincuded China.Hong Kong (hotel),and Hanoi (thepatientwastheindexpatientintheFrenchHospital).IsolationwasfromthekidneyonlylIsolationwasfromtheoropharyngealspecimenonly