Active transpor主动转运 口 Primary active transport原发性主动转运 口 Secondary active transport继发性主动转运
Active transport 主动转运 ❑Primary active transport 原发性主动转运 ❑Secondary active transport 继发性主动转运
Primary Active Transport making direct use of energy derived fro AtP to transport the ions across the cell menbrane
Primary Active Transport making direct use of energy derived from ATP to transport the ions across the cell membrane
The transported solute binds to the protein as it is phosphorylated (ATP expense Copyright e The McGraw-Hill Companies, Inc. Permission required for reproduction or displa 6 Extracellular fluid 3 Na+ High Na* Low K+ 3 Na+ ADP Intracellular fluid gh K- 。2K ATP
The transported solute binds to the protein as it is phosphorylated (ATP expense)
2 B ENZYMATIC CYCLE OF THE Na-K PUMP ree na’ion ell then bind to sites on Ey The E, form of the pump, with ATP bound, is empty/binding sites cellular space 8 3 An AtP molecule binds The ATP molecule is hydrolyze ge to the pump, which returns it in a conformational change, which its E, state. K is exposed occludes Na from both the to the cytosolic space and cytosolic and extracellular space. The conformation of the 7 pump spontaneously changes Phosphate leaves E, which changes its conformation Na to the extracellular space. once again and occludes K. allowing it to exit the pump. Ouabain inding Ouabain binds to ErP, changing the conform- ation of the pump and blocking the pore. The pump is nonfunctionaL. OUabain 6 the cell bind to sites The ErP form of pump is and exposed to the extracellular space
Concentration gradient of Nat and K+ Extracelluar(mmol/L) Intracellular(mmol/L) 140.0 15.0 4.0 150.0
Concentration gradient of Na+ and K+ Extracelluar (mmol/L) Intracellular (mmol/L) Na+ 140.0 15.0 K+ 4.0 150.0