Rich Media Are Required for Culture of animal cells o Nine amino acids referred to as the essential amino acids, cannot be synthesized by adult vertebrate animals and thus must be obtained from their diet animal cells grown in culture also must be supplied with these nine amino acids namely histidine, isoleucine leucine lysine valine addition, most cultured cells require cysteine, glutamine, and tyrosine o The other essential components of a medium for culturing animal cells are vitamins which the cell cannot make at all or in adequate amounts various salts glucose; and serum, the noncellular part of the blood
Rich Media Are Required for Culture of Animal Cells • Nine amino acids, referred to as the essential amino acids, cannot be synthesized by adult vertebrate animals and thus must be obtained from their diet. Animal cells grown in culture also must be supplied with these nine amino acids, namely, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine. • addition, most cultured cells require cysteine, glutamine, and tyrosine. • The other essential components of a medium for culturing animal cells are vitamins, which the cells cannot make at all or in adequate amounts; various salts; glucose; and serum, the noncellular part of the blood
Most cultured animal cells Grow Only on Special Solid Surfaces The extracellular matrices in various animal tissues consist of several common components; fibrous collagen proteins hyaluronan(or hyaluronic acid), a large mucopolysaccharide; and covalently linked polysaccharides and proteins in the form of proteoglycans(mostly carbohydrate) and glycoproteins(mostly protein)
Most Cultured Animal Cells Grow Only on Special Solid Surfaces • The extracellular matrices in various animal tissues consist of several common components: fibrous collagen proteins; hyaluronan (or hyaluronic acid), a large mucopolysaccharide; and covalently linked polysaccharides and proteins in the form of proteoglycans (mostly carbohydrate) and glycoproteins (mostly protein)
54体外细胞生长增殖过程 原代培养期 )传代培养期 )衰退期
5.4 体外细胞生长增殖过程 • 原代培养期 • 传代培养期 • 衰退期
55培养方法 )旋转管 )灌注小室 培养瓶 培养板
5.5 培养方法 • 悬滴 • 旋转管 • 灌注小室 • 培养瓶 • 培养板