5体外培养细胞基本技术 体外培养特点 体外培养工具 )外培养条件 不外细胞生长增殖过程 培养方法 大规模培养技术
5 体外培养细胞基本技术 • 体外培养特点 • 体外培养工具 • 体外培养条件 • 体外细胞生长增殖过程 • 培养方法 • 大规模培养技术
5,体外培养特点 营养条件苛刻 )适应性差,敏感 培养时间长,易污染
5.1 体外培养特点 • 营养条件苛刻 • 适应性差, 敏感 • 培养时间长，易污染
Animal cells are more difficult to culture than microorganisms because they require many more nutrients and typically grow only when attached to specially coated surfaces. Despite these difficulties, various types of animal cells including both undifferentiated and differentiated ones can be cultured successfully
• Animal cells are more difficult to culture than microorganisms because they require many more nutrients and typically grow only when attached to specially coated surfaces. Despite these difficulties, various types of animal cells, including both undifferentiated and differentiated ones, can be cultured successfully
5,2体外培养工具 空心纤维 做球
5.2 体外培养工具 • 空心纤维 • 微球
53体外培养条件 温度,37度 pH:7.27.4 )气体,氧,二氧化碳,氮气 养条件 需要多种氨基酸,维生素,辅酶,核酸 嘌呤,嘧啶,激素和生长因子,其中多种 成分可以由血清提供
5.3 体外培养条件 • 温度，37度 • pH：7.2-7.4 • 气体，氧，二氧化碳，氮气 • 营养条件 需要多种氨基酸，维生素，辅酶，核酸， 嘌呤，嘧啶，激素和生长因子，其中多种 成分可以由血清提供