How is a karyotype(核型 pl repared? Sample: blood, skin, amnio, cvs Cells grown in culture Blocked in metaphase using colchicine Spread on slide and stained Abnormalities detected by number, morphology and banding pattern
How is a karyotype（核型） prepared? • Sample: blood, skin, amnio, CVS • Cells grown in culture • Blocked in metaphase using colchicine • Spread on slide and stained • Abnormalities detected by number, morphology and banding pattern
XX XX XX XX XX XX XXXX XX XX XX 8I I XX Karyotype Analyse metaphase spread 5 ml venous blood igest with trypsin and st Add phytohemagglutin ith Giemsa and culture medium Culture at 37C Spread cells onto for 3 days slide by dropping Add colchicine and hypotonic saline Cells fixed
Centromere pracroemosome Anatom p(short arm) q (long arm) 3456 Size Banding pattern( giemsa stain) :昌翡異甾 Heterochromatin异染色质 789101112 (inactive, condensed)-dark Euchromatin常染色质 131415161718 (active, decondensed)-light 州s镇“ 19202122XY
Chromosome Anatomy • Centromere placement • p (short arm) • q (long arm) • Size • Banding pattern (Giemsa stain) – Heterochromatin 异染色质 (inactive, condensed) - dark – Euchromatin 常染色质 (active, decondensed) - light
Interphase metaphase nuclei Giemsa staining(G-banding)
Interphase & Metaphase Nuclei Giemsa Staining (G-banding)
Normal human male Karyotype Individual Chromosomes Cut from Metaphase Spread Hk义 )K#)台7 -】}
Normal Human Male Karyotype Individual Chromosomes Cut from Metaphase Spread