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生物 > 清华大学:《分子生物学》(英文版)Chapter 17 Rearrangement of DNA
清华大学:《分子生物学》(英文版)Chapter 17 Rearrangement of DNA
17.1 Introduction 17.2 The mating pathway is triggered by pheromone-receptor interactions 17.3 The mating response activates a G protein 17.4 Yeast can switch silent and active loci for mating type 17.5 The MAT locus codes for regulator proteins 17.6 Silent cassettes at HML and HMR are repressed 17.7 Unidirectional transposition is initiated by the recipient MAT locus 17.8 Regulation of HO expression 17.9 Trypanosomes switch the VSG frequently during infection 17.10 New VSG sequences are generated by gene switching 17.11 VSG genes have an unusual structure 17.12 The bacterial Ti plasmid causes crown gall disease in plants 17.13 T-DNA carries genes required for infection 17.14 Transfer of T-DNA resembles bacterial conjugation 17.15 Selection of amplified genomic sequences 17.16 Transfection introduces exogenous DNA into cells 17.17 Genes can be injected into animal eggs 17.18 ES cells can be incorporated into embryonic mice 17.19 Gene targeting allows genes to be replaced or knocked out
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17.5 Unidirectional transposition is initiated by the recipient mat locus Y region TTTCAGCTTTCCGCAACAGTATA Figure 17.9 HO AAAGTCGAAAGGCGTTGTCATAT endonuclear cleaves mat just to the right of the HO endonuclease Y region, generating sticky ends with a base TTTCAGCTTTCC GCAACA GTATA overhang AAAGTCGAAAGGCG TTGTCATAT 消当
Figure 17.9 HO endonuclease cleaves MAT just to the right of the Y region, generating sticky ends with a base overhang. 17.5 Unidirectional transposition is initiated by the recipient MAT locus
17.5 Unidirectional MAT transposition is initiated cut at Y boundary by the recipient mat ocus Pairing w ith dono Figure 17.10 Cassette Don HMR or HMAL substitution is initiated by a double-strand break in the Degradation of MAT recipient (MAT) locus, and may involve pairing on either side of the Y region with the Synthesis of new DNA donor(hmr or HML)locus Recipient dna has changed mating type 消当 Donor DNA is unchanged
Figure 17.10 Cassette substitution is initiated by a double-strand break in the recipient (MAT) locus, and may involve pairing on either side of the Y region with the donor (HMR or HML) locus. 17.5 Unidirectional transposition is initiated by the recipient MAT locus
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