7.91/7.36/BE490 Lecture #7 May4,2004 DNA Microarrays& Clustering Chris burge
7.91 / 7.36 / BE.490 Lecture #7 May 4, 2004 DNA Microarrays & Clustering Chris Burge
DNA Microarrays Clustering ° Why the hype? ° Microarray platforms CDNA VS oligo technologies Sample applications Analysis of microarray data clustering of co-expressed genes some classic microarray papers
DNA Microarrays & Clustering • Why the hype? • Microarray platforms - cDNA vs oligo technologies • Sample applications • Analysis of microarray data - clustering of co-expressed genes - some classic microarray papers
Stanford U. Dept. of Biochemistry Web site tanford University.: DEPARTMENT OF Biochemistry http://cmgm.stanford.edu/biochem/
Stanford U. Dept. of Biochemistry Web Site http://cmgm.stanford.edu/biochem/
Why microarrays? Changes in gene expression are important in many biological contexts Development Cancer other Diseases Environmental Adaptation DNA microarrays provide a high throughput way to study these changes
Why Microarrays? • Changes in gene expression are important in many biological contexts: – Development – Cancer – Other Diseases – Environmental Adaptation • DNA microarrays provide a high throughput way to study these changes
What's new? progression to chip technology Hybrid detection radioactive labeling fluorescent labeling Solid support for sample fixation Southern blots northern blots etc Main advantage of microarrays is scale Probes are attached to solid support Efficient robotics Bioinformatic analysis Parallel measurement of thousands of genes at a time
What’s new? … progression to chip technology • Hybrid detection – radioactive labeling – fluorescent labeling • Solid support for sample fixation – Southern blots, Northern blots, etc. • Main advantage of microarrays is scale – Probes are attached to solid support – Efficient robotics – Bioinformatic analysis • Parallel measurement of thousands of genes at a time