Figure 6.6 A Millipore Membrane Filter System. (a)Parts of the filter assembly.Some of the more important components are the following:(1)funnel, (2)membrane filter,(3)filter holder base and support,and(4)receiver flask.(b)A complete filtering system.The sample is poured into the funnel and sucked through a membrane filter with the use of vacuum. 3、膜过滤培养法 Membrane filter emoved and placed in plate Water sample filtered containing the Membrane filte through membrane appropriate Incubation on a filter support filter (0.45 um) medium Typical for 24 hours coliform colonies Figure 6.7 The Membrane Filtration Procedure
3、膜过滤培养法
样品中菌数很低：湖水、海水或饮用水 Figure 6.17 Counting bacteria by filtration.(a)The bacteria in 100 ml of water were sieved out onto the surface of a membrane filter.(b)Such a filter,with the bacteria much more widely spaced, was placed on a pad saturated with liquid nutrient medium,and the individual bacteria grew into visible colonies.One hundred twenty four colonies are visible,so we would record 124 bacteria per 100 ml of water sample. Bacteria can be counted by filtra- tion when their quantity is very small. (b) (a) SEM 2μm
样品中菌数很低：湖水、海水或饮用水
南极深处湖泊的细菌
南极深处湖泊的细菌
4、比浊法 在一定波长下测定菌悬 中 液的光密度，以光密度 Am A Single So (optical density,即0.D.) 表示菌量。 numbers or differentate between dead and live cell
4、比浊法 在一定波长下测定菌悬 液的光密度，以光密度 (optical density, 即O.D.) 表示菌量
(二) 重量法 千重、湿重 通过样品中蛋白质、核酸含量的测定间接 推算微生物群体的生物量 适于测定多细胞及丝状真菌的生长情况
(二) 重量法 干重、湿重 适于测定多细胞及丝状真菌的生长情况 通过样品中蛋白质、核酸含量的测定间接 推算微生物群体的生物量