II. pathogenesis of ischemia-reperfusion Injury 1. The role of free radical (1 kinds and concept of free radical free radical:
Ⅱ. pathogenesis of ischemia-reperfusion injury 1. The role of free radical ⑴ kinds and concept of free radical free radical:
normal: 0+4e+4h. 2HO e-+2H+ o e,0 B,0 e+H+ e+h+ 一OH HO HO
normal: O2 +4e+4H+ 2H2O O2 →O - • 2 H2O2 OH • H2O e - e - + 2H + e - + H + e - + H + H2O
oxvgen free radical OH° kinds of (active oxygen: O2H2O2 OH) free radical li oid free radical: pi L、LO°、LOO others:Cr、CH3、NO
oxygen free radical: O - • 2 、OH • kinds of (active oxygen: 1O2、H2O2 OH • ) free radical lipid free radical: L• 、LO• 、LOO• others: Cl• 、CH3 • 、NO
(2) mechanism of increase of oxygen free radical O formation of oxygen free radical nature oxidation of Hb, Cyt c O,→0,-H,O,=OH-2O HO oxidation of enzyme: xanthine oxidase(Xo) xanthine uric acid OO Mitochondria:o 2
(2) mechanism of increase of oxygen free radical ① formation of oxygen free radical nature oxidation of Hb , Cyt C O2 O ‾∙ 2 H2O2 OH∙ H2O H2O oxidation of enzyme : xanthine oxidase(XO) O ‾∙ 2 xanthine uric acid O2 O ‾∙ 2 Mitochondria: O ‾∙ 2