华大生命科学研究院单细胞序列比对的迷思BGl-ResearchTSSExonTSSExonIntronExonIntron(a)MapMap againMMP 1MMP 215°-3RNA-seg readHHHHH工Hexons in the genomeMappedtogenome(b)(c)MapMapCDS5'capPolyAMMP 1ExtendMMP1Trim5'3'A-tail, or adapter,mismatchesHHHHHHorpoorqualitytailHHHHHHReadsFastQFastQFastQMappedtotranscriptomeVWWMappingSTARHiSat2[Kallisto]Salmonl10xBarcodePoly(dTIVNQuantificationfeatureCountsStringTieVVR1UMIDifferentialDESeq2/SleuthBallgownedgeR/geneexpressionLimma
单细胞序列比对的迷思
华大生命科学研究院RNA定量与参考序列的优化BGI-Research.Read counts=geneexpressionUnion.ReadscanbequantifiedonanyfeatureReadGeneA(gene,transcript,exonetc)Gene.IntersectionongenemodelsGeneA.Gene/Transcript levelGeneA吕二GeneAGeneCGeneAGeneBnaturemethodsGeneAExplore content vAboutthe journal PublishwithusvAmbiguousnature > nature methods > articles > articleBArticlePublished:11September2023Recoveryofmissingsingle-cellRNA-sequencingdataAmbiguouswithoptimizedtranscriptomicreferencesBAllaoensttnkAmbiguousBAMature Methods (2023) /Cite this article3722Accesses89AltmetricMetrics
RNA定量与参考序列的优化