Large ribosomal subunit, 9A resolution 5S rRNA region L7几12 Ban,. Moore, steitz Cell.Vol.93.1105-1115 1998 Ittp://www.life.nthu edu. tw /-b830356/ribosome 100A e. html 55 TRNA region 5s rRNA regi L712 L7几L1
Large ribosomal subunit, 9 A resolution http://www.life.nthu .edu.tw /~b830356/ribosom e.html Ban, ..Moore, Steitz, Cell, Vol. 93, 1105-1115, 1998
30s ribs subunit from side that contacts the 50s subunit http://chem-faculty.ucsdedu/joseph/ 50S ribs subunit from side that Ribosome_ Structure_ Gallery. html Schluenzenet al. Cell (2000)102, 615-623 contacts the 30s subunit Ban,. P. Moore, T Steitz; Science(2000)289 905-920
3-D views of ribosome subunits http://chem-faculty.ucsd.edu/joseph/ Ribosome_Structure_Gallery.html Schluenzen et al. Cell (2000) 102, 615-623 Ban, ...P. Moore, T. Steitz; Science (2000) 289 905-920 30S ribs subunit, from side that contacts the 50S subunit 50S ribs subunit, from side that contacts the 30S subunit
核糖体结合位点 SD sequence Ribosome binding site(rbs) ·在原核生物mRNA起始密码子上游8-13个核苷酸 处,通常有5- AGGAGGU-3的富含嘌呤的保守序 列,这段序列可与核糖体16 SrrNA的3端序列5 ACCUCCU-3进行碱基配对,因此成为核糖体结 合位点 Shine-Dalgarno序列或SD序列 ·SD序列的作用是相对于起始密码子而正确定位核 糖体 G GUAUG mRNA 3 AUCCUCCA 16S rrNA
核糖体结合位点 SD sequence Ribosome binding site (RBS) • 在原核生物mRNA起始密码子上游8-13个核苷酸 处，通常有5’-AGGAGGU-3’的富含嘌呤的保守序 列，这段序列可与核糖体16S rRNA的3’端序列5’- ACCUCCU-3’ 进行碱基配对，因此成为核糖体结 合位点Shine-Dalgarno序列或SD序列 • SD序列的作用是相对于起始密码子而正确定位核 糖体 GUAGGAGGUGUAUG mRNA 3’ 16S rRNA AUCCUCCA
Function: For the position of ribosome correctly with respect to the initiation codon E coli trp A 5)AGC CGAGGGGAAAUCUGAU GGAACGCUA C(3) E coli araB U UUGGA UGGAGUGAAACGAUGGCGAUUGCA E coli lacI CAA UUCAGGGUGGUGAAUGUGAAACCAGUA oX174 phage A protein AA UCUUGGAGGCUUUUUUAUGGUUCGUUCU a phage cro AU GUACUAAGGAGGUUGUAUGGAACAACGC Shine- Dalgarno sequence; Initiation codon pairs with 16srRNA pairs with fMet-tRNAIMet G 3’ End of Prokaryotic mrNA 16S rRNA with consensus UCCUCCA Shine-Dalgarno (5)GA UUCCUAGGAGGUUUGACCUAUGCGAGCUU U UA G U(3) sequence (b)
AUG Function: For the position of ribosome correctly with respect to the initiation codon
Translation in procaryotes 原核生物的翻译可以分为三个阶段 initiation起始 elongation延长 termination终止 每一步都需要一些特定的蛋白质分子参与和 trNas,mRNA,and/ or ribosomes的相互作用
原核生物的翻译可以分为三个阶段 • initiation起始 • elongation延长 • termination终止 每一步都需要一些特定的蛋白质分子参与和 tRNAs, mRNA, and/or ribosomes的相互作用 Translation in procaryotes