g.ADP ADP App(NHP B-ADP ADP ATP a-empty (b) (c) s Empty Rod-shaped y subunit Each b sununit of ATP synthase can assume three different conformations
Rod-shaped g subunit. ADP App(NH)p Empty Each b sununit of ATP synthase can assume three different conformations!
6. The binding-change model was proposed to explain the action mechanism of ATP synthase The model was proposed by paul Boyer in 1973 (PNAS, 70: 2837-2839), based on kinetic and binding studies(before the 3-D structure of bovine Fior yeast FoF was determined) Downhill proton movement through Fo will drive the rotation of the c-subunit ring and the asymmetrical γ subunits, which will cause each of the threeβ subunits to interconvert between the three take up adp+Pi synthesize ATP, and release ATP o conformations. as a result. each of them take turns to
16. The binding-change model was proposed to explain the action mechanism of ATP synthase • The model was proposed by Paul Boyer in 1973 (PNAS, 70:2837-2839), based on kinetic and binding studies (before the 3-D structure of bovine F1 or yeast FoF1 was determined). • Downhill proton movement through Fo will drive the rotation of the c-subunit ring and the asymmetrical g subunits, which will cause each of the three b subunits to interconvert between the three conformations, as a result, each of them take turns to take up ADP + Pi , synthesize ATP, and release ATP
Rotations of the y subunit and the c subunits of the FI unit in three discrete steps of 1200(powered by aTP hydrolysis catalyzed by the p subunits) have been directly observed using fluorescence microscopy by Dr Kazuhiko Kinosita in 1997 ( Nature,386:299-302) The estimation of Ht consumption for each ATP formed is 4(among which one is consumed for P transport), thus about 2.5 ATP/NADH, 1.5 ATP/FADH The chemiosmotic coupling allows nonintegral stoichiometries of O2 consumption (or NADH and FADH, oxidation) and atP synthesis
• Rotations of the g subunit and the c subunits of the F1 unit in three discrete steps of 120o (powered by ATP hydrolysis catalyzed by the b subunits) have been directly observed using fluorescence microscopy by Dr. Kazuhiko Kinosita in 1997 (Nature, 386:299-302) . • The estimation of H+ consumption for each ATP formed is 4 (among which one is consumed for Pi transport), thus about 2.5 ATP/NADH, 1.5 ATP/FADH2 . • The chemiosmotic coupling allows nonintegral stoichiometries of O2 consumption (or NADH and FADH2 oxidation) and ATP synthesis
a B ATP B-ATP The binding-change B ADP P model proposed by aul Boyer 3 Hp 3 ATP 3 HN 3 Hp ATP BBer mptv ATP BiB-ApP ADP +P BATE B ADP +P ATP 3 Hp 3 HN
b-ATP b-empty b-ADP The binding-change model proposed by Paul Boyer g g g
5o8948082889 Rota tion of the Y Actin filament subunit and the Avidin ring of c subunits in the FoFi complex was observed by in vitro studies using fluorescence microscopy F aDP +P B ATP Hi residues His residues Ni complex
Rotation of the g subunit and the ring of c subunits in the FoF1 complex was observed by in vitro studies using fluorescence microscopy