PhaseMicroscopeNikonPhase ContrastandInterferenceAccessory(circa 1970s)SHIHEZI UNIVERSITY
Phase Microscope SHIHEZI UNIVERSITY SHIHEZI UNIVERSITY
Fluorescent MicroscopeSHIHEZI UNIVERSITY
Fluorescent Microscope SHIHEZI UNIVERSITY SHIHEZI UNIVERSITY
Confocal MicroscopeDetectorDetector-PinholeApertureOut-of-FocusLight RaysDichromaticLaserMirrorExcitationIn-Focus-SourceLight RaysExcitationLightObjectiveRaysLight SourcePinholeApertureFocalPlanesSpecimenSHIHEZI UNIVERSITY
Confocal Microscope SHIHEZI UNIVERSITY SHIHEZI UNIVERSITY
Stain? Gram Stain? Acid-fast stain· Stains for special structuresSHIHEZI UNIVERSITY
Stain •Gram Stain •Acid-fast stain •Stains for special structures SHIHEZI UNIVERSITY SHIHEZI UNIVERSITY
Staining Reaction· Stains - salts composed of a positive andnegative on, one of which is colored(chromophore)? Basic Dyes - chromophore is the positive ion- dye+ Cl-: Acid Dyes - chromophore is the negative ion- Na+ dye-SHIHEZI UNIVERSITY
Staining Reaction •Stains - salts composed of a positive and negative ion, one of which is colored (chromophore) •Basic Dyes - chromophore is the positive ion – dye+ Cl- • Acid Dyes - chromophore is the negative ion – Na+ dyeSHIHEZI UNIVERSITY SHIHEZI UNIVERSITY