《病原生物学（医学微生物学）》课程教学课件（英文）01_BacterialStructure

Optical Observation Methodologyfor MicroorganismsLight MicroscopeDarkfield MicroscopePhase MicroscopeFluorescent MicroscopeConfocal MicroscopeElectron MicroscopeAutoradiographySHIHEZI UNIVERSITY

Optical Observation Methodology for Microorganisms Light Microscope Darkfield Microscope Phase Microscope Fluorescent Microscope Confocal Microscope Electron Microscope Autoradiography SHIHEZI UNIVERSITY SHIHEZI UNIVERSITY

Microscope Resolving Power Ability to distinguish two distinct points: Absolute limit of the Resolving Power isabout 1/2 the wavelength of light that isused to illuminate the specimenwavelength = 2nm- e.g.Resolution limit = InmSHIHEZI UNIVERSITY

Microscope Resolving Power •Ability to distinguish two distinct points •Absolute limit of the Resolving Power is about 1/2 the wavelength of light that is used to illuminate the specimen –e.g. wavelength = 2nm Resolution limit = 1nm SHIHEZI UNIVERSITY SHIHEZI UNIVERSITY

Light MicroscopeResolution limit: 0.1 μmMagnification options:100X400X1000XSHIHEZI UNIVERSITY

Light Microscope Resolution limit: 0.1 m Magnification options: 100X 400X 1000X SHIHEZI UNIVERSITY SHIHEZI UNIVERSITY

MicroscopeElectronTransmission EMScanning EMSHIHEZI UNIVERSITY

Electron Microscope Scanning EM Transmission EM SHIHEZI UNIVERSITY SHIHEZI UNIVERSITY

Darkfield MicroscopeSpot Ring Bicentric CondenserHollowConeofluminatior(NA = 1.50)MirroredSurfaceSolidGlassDesignLightMiroredFromSurfaceSourceFigure 1SeatleSTDHVPreventionTaining CenerSource:UniversityofWashingtan

Darkfield Microscope