Complex viruses 65mm一 Capsid(head) Some viruses, particularly bacterial viruses, have very complicated structures and are called complex DNA viruses. Examples of complex viruses are poxviruses. which do not contain clearly identifiable capsids sheath but have several coats around the nucleic acid. Certain bacteriophages have capsids to which additional Endplate structures are attached -Tail fibers
Some viruses, particularly bacterial viruses, have very complicated structures and are called complex viruses. Examples of complex viruses are poxviruses, which do not contain clearly identifiable capsids but have several coats around the nucleic acid. Certain bacteriophages have capsids to which additional structures are attached. Complex viruses
Capid hate Tai fiber A virus can have either dna or rna but never both !
A virus can have either DNA or RNA but never both !!
6.3 Cultivating Viruses Bacteriophage: bacterial Cultures Animal viruses: Tissue or cell cultures Plant viruses: tissue or separated cell cultures, or cultures of protoplasts
6.3 Cultivating Viruses • Bacteriophage: bacterial Cultures • Animal viruses: Tissue or cell cultures • Plant viruses: tissue or separated cell cultures, or cultures of protoplasts
Quantification of viruses m Bactenal Ce top ac nutr Plaque assay technique for quantification of Lawn of host cells bacterial viruses
Quantification of viruses • Plaque assay technique for quantification of bacterial viruses
Quantification of bacterial virus by plaque assay Quantification of a Bacterial virus by Plaque assay 2. The mixture poured on the surface of a nutrient agar plate Molten 3. The host bacteria begin Bacterial cells to grow, and after overnight Pour mixture onto ne pg plpe incubation form a lawn of confluent growth Nutrient agar plate Sandwich of top agar and Lawn of host cells nutrient ngar 1. a dilution of a suspension containing the virus material is mixed in a small amount of melted agar with the sensitive host bacteria
1. A dilution of a suspension containing the virus material is mixed in a small amount of melted agar with the sensitive host bacteria. 3. The host bacteria begin to grow, and after overnight incubation form a lawn of confluent growth. Quantification of bacterial virus by plaque assay 2. The mixture poured on the surface of a nutrient agar plate