21 plate colony- counting methods(平板菌落计数法 The method is suitable for aerobic and anerobic microbes, every living cell will form a colony forming unit (菌落形成单位cu), the total number of living cell in the sample can be calculated from cfu X dilution factor(PA 倍数) mr()平
2.1 plate colony-counting methods(平板菌落计数法) The method is suitable for aerobic and anearobic microbes, every living cell will form a colony forming unit (菌落形成单位cfu), the total number of living cell in the sample can be calculated from cfu × dilution factor(稀释 倍数). pour plate (浇注平板) spread plate (涂布平板)
1ml :10 225m无菌 1ml 试管中原始装 9m无菌水 水加25g样品
225ml无菌 水加25g样品 1ml 1ml 1ml 试管中原始装 9ml无菌水 10-2 10-3 10-4 1ml
2.2 anaerobic colony-counting method Hungate roll-tube technique(亨盖特滚管技术)is designed by famous American microbiologist R E Hungate in 1950. it's a epoch-making invention in the development history of microbiology which made the isolation and study for the obligate anerobic possible The method above needs complicated equipment and the skillful technique. Zhou deqing invented semi-solid media deep agar method(半固体深层琼脂法) which is easily manipulated
2.2 anaerobic colony-counting method Hungate roll-tube technique (亨盖特滚管技术)is designed by famous American microbiologist R. E. Hungate in 1950. it’s a epoch-making invention in the development history of microbiology which made the isolation and study for the obligate anearobic possible. The method above needs complicated equipment and the skillful technique. Zhou deqing invented semi-solid media deep agar method(半固体深层琼脂法)which is easily manipulated
Section B Rules of microbial growth PartA cell growth and synchronous growth Part B typical growth curve of unicelled microbe Part c continuous culture Part d high cell-density culture
Section B Rules of microbial growth Part A cell growth and synchronous growth Part B typical growth curve of unicelled microbe Part C continuous culture Part D high cell-density culture
Part A cell growth and synchronous growth Extremely complicated biochemical changes and cytological changes are going on in the tiny cells periodical along with the cell growth, the observation of these changes re fairly difficult. The methods used present are 1. Ultra- thin section ea(电镜观察超薄切片) 2. Synchronous culture(同步培养技术)
Part A cell growth and synchronous growth Extremely complicated biochemical changes and cytological changes are going on in the tiny cells periodically along with the cell growth, the observation of these changes are fairly difficult. The methods used present are : 1. Ultra-thin section EM (电镜观察超薄切片) 2. Synchronous culture (同步培养技术)